Ni-NTA Agarose
Product characteristics | |
|---|---|
| Matrix | 6 % cross-linked agarose |
| Bead size | 50-170 µm |
| Linker | Nitrilotriacetic acid (NTA) |
| Binding capacity* | > 50 mg/ml gel |
| Recommended flow rate | 1 ml column – 1.0 ml/min 10 ml column – 5.0 ml/min |
| Maximum linear flow rate | 26 cm/h |
| Maximum pressure | 2.8 psi |
| Recommended pH | between 7 - 8 |
| Chemical stability | Stable to all solutions commonly used in gel filtration including reducing agents (see procedure) |