Detecção por EvaGreen para Genotipagem/ High Resolution Melt (HRM)
JBS qPCR GreenMaster Series is designed for quantitative real-time analysis of DNA samples including High Resolution Melting (HRM) curve analysis. The mixes contain all reagents required for qPCR (except template and primers) in a premixed 2x concentrated ready-to-use solution. The mixes are recommended for routine PCR applications, high throughput PCR or genotyping and provide an improved specificity and sensitivity when amplifying low-copy-number targets or working with complex backgrounds.
The fluorescent DNA stain EvaGreen® intercalates into the amplification product during the PCR process and allows the direct quantification of target DNA without the need of sequence-specific labeled probes (i.g. TaqMan® Probes).
High Resolution Melting (HRM) analysis is a powerful tool for detection of mutations, polymorphisms and epigenetic differences in DNA samples. It is a fast and cost effective alternative to other genotyping technologies.
UNG (Uracil-N-Glycosylase) eliminates carry-over contamination of DNA from previous PCR reactions. An UNG treatment at the onset of thermal cycling removes uracil residues from dU-containing DNA and prevents it from serving as template.
ROX reference dye does not take part in the PCR reaction but allows to normalize for non-PCR related signal variation.
EvaGreen® Fluorescent DNA Stain is a DNA intercalator dye specially developed for DNA analysis applications including real-time PCR (qPCR) and high-resolution DNA melting curve analysis (HRM). Upon binding to DNA, the non-fluorescent dye becomes highly fluorescent while showing no detectable inhibition to the PCR process. The dye is extremely stable both thermally and hydrolytically, providing convenience during routine handling.
qPCR Green Master 2x PCK-100
Master mix para real-time PCR com corante de DNA EvaGreen verde fluorescente
qPCR Green Master Low ROX 2x PCK-101
Master mix para real-time PCR com EvaGreen fluorescente e low ROX
qPCR GoldMaster PCK-200
Master mix para real-time PCR com corante de DNA EvaGreen e GoldTaq hot start polimerase quimicamente modificada
SYBR® Green é uma marca registrada pela Invitrogen Corporation, Carlsbad, California, USA.