Nucleotídeos e Nucleosídeos

Nucleotídeos Fluorescentes

Seletor de Nucleotídeos Fluorescentes

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Fluorescent Dyes and Quenchers - Properties and Structures

Fluorescent Nucleotides

Adenosinas – Marcador Reporter

Adenosinas – Marcador Quencher

Adenosinas – Fluorescência Intrínseca

Guanosinas – Marcador Reporter

Guanosinas – Marcador Quencher

Guanosinas – Fluorescência Intrínseca

Citosinas – Marcador Reporter

Citosinas – Marcador Quencher

Citosinas – Fluorescência Intrínseca

Uridinas – Marcador Reporter

Uridinas – Marcador Quencher

Uridinas – Fluorescência Intrínseca

Xantosinas e Inosinas – Fluorescência Intrínseca

Nucleotídeos - Marcadores Distintos

Table 1: Enzymatic incorporation of fluorescent Nucleotides.

PCR: Polymerase chain reaction with Taq Polymerase; MDA-based WGA: Multiple displacement amplification (MDA)-based whole genome amplification (WGA) with Phi29 DNA Polymerase; NT: Nick Translation with DNAse I / DNA Polymerase I; Primer Extension: Primer Extension with Klenow 3'-5'exo-; 3'-End Labeling: Incorporation with Terminal deoxynucleotidyl Transferase (TdT); RT: Reverse Transcription with Moloney Murine Leukemia Virus Reverse Transcriptase (MMLV RT)

			DNA Labeling				cDNA Labeling
Emission color	Nucleotide	Cat. No.	PCR	NT	Primer Extension	3' End Labeling	RT
dUTP
blue	AMCA-dUTP	NU-803-AMCA	-	✔²	✔²	n/a	n/a
	DEAC-dUTP	NU-803-DEAC	-	✔¹	n/a	n/a	n/a
	dUTP-ATTO-425	NU-803-425	✔¹	✔^1,2	✔¹	✔¹	n/a
	dUTP-XX-ATTO-425	NU-803-XX-425	✔¹	✔¹	n/a	n/a	n/a
green	dUTP-XX-AF488	NU-803-XX-AF488	✔¹	✔¹	n/a	n/a	n/a
	dUTP-XX-ATTO-488	NU-803-XX-488	✔^1,2	✔^1,2	✔^1,2	✔^1,2	n/a
	Fluorescein-12-dUTP	NU-803-FAMX	✔^1,2	✔^1,2	✔^1,2	✔^1,2	n/a
	Rhodamine-12-dUTP	NU-803-RHOX	-	✔^1,2	✔¹	✔^1,2	n/a
yellow- green	dUTP-XX-ATTO-532	NU-803-532	✔¹	✔¹	✔¹	✔¹	n/a
yellow	dUTP-Cy3	NU-803-Cy3	✔^1,2	✔^1,2	✔¹	✔¹	✔²
	dUTP-XX-Cy3	NU-803-XX-CY3	✔¹	✔¹	✔¹	n/a	✔¹
	dUTP-XX-AF555	NU-803-XX-AF555	✔¹	✔¹	n/a	n/a	n/a
	dUTP-ATTO-550	NU-803-550	-	✔¹	✔¹	✔¹	n/a
orange	dUTP-Texas Red	NU-803-TXR	✔^1,2	✔^1,2	✔²	n/a	n/a
	dUTP-XX-Texas Red	NU-803-XX-TXR	✔^1,2	✔^1,2	✔²	n/a	n/a
	dUTP-XX-AF594	NU-803-XX-AF594	✔^1,2	✔^1,2	✔¹	n/a	n/a
	dUTP-XX-594	NU-803-XX-594	✔¹	✔¹	✔¹
red	dUTP-Cy5	NU-803-CY5	✔^1,2	✔^1,2	✔¹	n/a	✔²
	dUTP-XX-Cy5	NU-803-XX-CY5	✔¹	✔¹	✔¹	n/a	✔¹
	dUTP-ATTO-647N	NU-803-647N	-	✔^1,2	✔¹	✔¹	n/a
	dUTP-ATTO-643	NU-803-643	✔¹	✔¹	✔¹
	dUTP-XX-ATTO-643	NU-803-XX-643	✔¹	✔¹	✔¹
	dUTP-XX-AF647	NU-803-XX-AF647	✔¹	✔¹	n/a	n/a	n/a
	dUTP-XX-STAR RED	NU-803-XX-STRED	✔¹	n/a	n/a	n/a	n/a
	dUTP-ATTO-655	NU-803-655	-	✔¹	✔¹	✔¹	n/a
IR-red	dUTP-XX-IR680LT	NU-803-XX-IR680LT	✔¹	n/a	n/a	n/a	n/a
IR-red	dUTP-XX-IR750	NU-803-XX-IR750	✔¹	n/a	n/a	n/a	n/a
dCTP
green	Fluorescein-12-dCTP	NU-809-FAMX	✔¹	✔¹	n/a	n/a	n/a
green	Rhodamine-12-dCTP	NU-809-RHOX	-	✔¹	n/a	n/a	n/a
yellow	dCTP-Cy3	NU-809-Cy3	✔¹	✔¹	n/a	n/a	n/a
yellow	dCTP-ATTO-550	NU-809-550	-	✔¹	n/a	n/a	n/a
orange	dCTP-Texas Red	NU-809-TXR	✔¹	✔¹	n/a	n/a	n/a
red	dCTP-Cy5	NU-809-CY5	✔¹	✔¹	n/a	n/a	n/a
	dCTP-ATTO-647N	NU-809-647N	-	✔¹	n/a	n/a	n/a

¹: in-house tested ²: external reference (see datasheet) n/a: no data available - : not applicable