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Nucleotídeos Fluorescentes

Seletor de Nucleotídeos Fluorescentes

Table 1: Enzymatic incorporation of fluorescent Nucleotides.

PCR: Polymerase chain reaction with Taq Polymerase; MDA-based WGA: Multiple displacement amplification (MDA)-based whole genome amplification (WGA) with Phi29 DNA Polymerase; NT: Nick Translation with DNAse I / DNA Polymerase I; Primer Extension: Primer Extension with Klenow 3'-5'exo-; 3'-End Labeling: Incorporation with Terminal deoxynucleotidyl Transferase (TdT); RT: Reverse Transcription with Moloney Murine Leukemia Virus Reverse Transcriptase (MMLV RT)

DNA Labeling cDNA
Labeling
Emission
color		NucleotideCat. No.PCR NTPrimer Extension3' End LabelingRT
dUTP
blueAMCA-dUTPNU-803-AMCA-✔2✔2n/an/a
DEAC-dUTPNU-803-DEAC-✔1n/an/an/a
dUTP-ATTO-425NU-803-425✔1✔1,2✔1✔1n/a
dUTP-XX-ATTO-425NU-803-XX-425✔1✔1n/an/an/a
greendUTP-XX-AF488NU-803-XX-AF488✔1✔1n/an/an/a
dUTP-XX-ATTO-488NU-803-XX-488✔1,2✔1,2✔1,2✔1,2n/a
Fluorescein-12-dUTPNU-803-FAMX✔1,2✔1,2✔1,2✔1,2n/a
Rhodamine-12-dUTPNU-803-RHOX-✔1,2✔1✔1,2n/a
yellow-
green		dUTP-XX-ATTO-532NU-803-532✔1✔1✔1✔1n/a
yellowdUTP-Cy3NU-803-Cy3✔1,2✔1,2✔1✔1✔2
dUTP-XX-Cy3NU-803-XX-CY3✔1✔1✔1n/a✔1
dUTP-XX-AF555NU-803-XX-AF555✔1✔1n/an/an/a
dUTP-ATTO-550NU-803-550-✔1✔1✔1n/a
orangedUTP-Texas RedNU-803-TXR✔1,2✔1,2✔2n/an/a
dUTP-XX-Texas RedNU-803-XX-TXR✔1,2✔1,2✔2n/an/a
dUTP-XX-AF594NU-803-XX-AF594✔1,2✔1,2✔1n/an/a
dUTP-XX-594NU-803-XX-594✔1✔1✔1
reddUTP-Cy5NU-803-CY5✔1,2✔1,2✔1n/a✔2
dUTP-XX-Cy5NU-803-XX-CY5✔1✔1✔1n/a✔1
dUTP-ATTO-647NNU-803-647N-✔1,2✔1✔1n/a
dUTP-ATTO-643NU-803-643✔1✔1✔1
dUTP-XX-ATTO-643NU-803-XX-643✔1✔1✔1
dUTP-XX-AF647NU-803-XX-AF647✔1✔1n/an/an/a
dUTP-XX-STAR REDNU-803-XX-STRED✔1n/an/an/an/a
dUTP-ATTO-655NU-803-655-✔1✔1✔1n/a
IR-reddUTP-XX-IR680LTNU-803-XX-IR680LT✔1n/an/an/an/a
dUTP-XX-IR750NU-803-XX-IR750✔1n/an/an/an/a
dCTP
greenFluorescein-12-dCTPNU-809-FAMX✔1✔1n/an/an/a
Rhodamine-12-dCTPNU-809-RHOX-✔1n/an/an/a
yellowdCTP-Cy3NU-809-Cy3✔1✔1n/an/an/a
dCTP-ATTO-550NU-809-550-✔1n/an/an/a
orangedCTP-Texas RedNU-809-TXR✔1✔1n/an/an/a
reddCTP-Cy5NU-809-CY5✔1✔1n/an/an/a
dCTP-ATTO-647NNU-809-647N-✔1n/an/an/a
1: in-house tested 2: external reference (see datasheet) n/a: no data available - : not applicable
Adenosinas – Marcador Reporter
Adenosinas – Marcador Quencher
Adenosinas – Fluorescência Intrínseca
Guanosinas – Marcador Reporter
Guanosinas – Marcador Quencher
Guanosinas – Fluorescência Intrínseca
Citosinas – Marcador Reporter
Citosinas – Marcador Quencher
Citosinas – Fluorescência Intrínseca
Uridinas – Marcador Reporter
Uridinas – Marcador Quencher
Uridinas – Fluorescência Intrínseca
Xantosinas e Inosinas – Fluorescência Intrínseca
Nucleotídeos - Marcadores Distintos