Purificação de Proteínas His-tagged

Selected References

[1] Porath et al. (1975) Metal chelate affinity chromatography, a new approach to protein fractionation. Nature 258:598.
[2] Gaberc-Porekar et al. (2001) Perspectives of immobilized-metal affinity chromatography. J. Biochem. Biophys. Methods 49:335.
[3] Ueda et al. (2003) Current and prospective applications of metal ion–protein binding. Journal of Chromatography 988:1.
[4] Liesiene et al. (1997) Immobilized metal affinity chromatography of human growth hormone-effect of ligand density. Journal of Chromatography 764:27.