Glutationa Agarose
Glutathione Agarose characteristics | |||
|---|---|---|---|
| Matrix | 4 % agarose | ||
| Linker | 12-atom spacer | ||
| Bead size | 50 - 150 µm | ||
| Binding capacity* | > 8 mg/ml resin | ||
| Max. linear flow rate | 250 cm/h | ||
| pH stability | 2 - 12 | ||
| Chemical stability | stable in 0.1 M acetate pH 4.0, 0.1 M NaOH, 70 % ethanol or 6 M guanidine hydrochloride for two hours at room temperature, not autoclavable | ||
| Recommended flow rates | |||
bed volume | inner diameter | sample loading | washing/elution |
1 ml | 6.6 mm | 0.2 - 1.0 ml/min | 1.0 ml/min |
10 ml | 16 mm | 0.5 - 5.0 ml/min | 5.0 ml/min |
* tested with human Glutathione S-transferase (GST)
Selected References
[1] Bee et al. (2010) Growth and tumor suppressor NORE1A is a regulatory node between Ras signaling and microtubule nucleation. J. Biol. Chem. 285 (21):16258.