3TCTP

Lamivudine triphosphate, Sodium Salt (L-Isomer)

2', 3'-Dideoxy-3'-thia-cytidine-5'-triphosphate, Sodium salt (L isomer)

Catálogo Nº	Apresentação	Preço (R$)	Comprar
NU-1606S	5 μl (10 mM)	Sob demanda	Adicionar ao Carrinho
NU-1606L	5 x 5 μl (10 mM)	Sob demanda	Adicionar ao Carrinho

For general laboratory use.

Envio: shipped on gel packs

Condições de armazenamento: store at -20 °C
Short term exposure (up to 1 week cumulative) to ambient temperature possible.

Validade: 12 months after date of delivery

Fórmula molecular: C8H14N3O12P3S (free acid)

Peso molecular: 469.19 g/mol (free acid)

CAS#: 143188-53-8

Pureza: ≥ 95 % (HPLC)

Forma: solution in water

Concentração: 10 mM - 11 mM

pH: 7.5 ±0.5

Propriedades espectroscópicas: λabs 270 nm, ε 9.5 L mmol-1 cm-1 (Tris-HCl pH 7.5)

Formulários:
Monitoring in mononuclear cells by ion-pair LC and MS^[1]
Influence on incorporation efficiency^[2]
Inhibition of DNA/RNA-dependent DNA-polymerase^[3]

Referências selecionadas:
[1] Coulier et al. (2008) Simultaneous determination of endogenous deoxynucleotides and phosphorylated nucleoside reverse transcriptase inhibitors in peripheral blood mononuclear cells using ion-pair liquid chromatography coupled to mass spectrometry. Proteomics Clin. Appl. 2:1557.

[2] Lim et al. (2001) Differential incorporation and removal of antiviral deoxynucleotides by human DNA polymerase y. J. Biol. Chem. 276 (26):23616.

[3] Hart et al. (1992) Effects of (-)-2-deoxy-3-thiacytidine (3TC) 5-triphosphate on human immunodeficiency virus reverse transcriptase and mammalian DNA polymerases alpha, beta and gamma. Antimicrobial Agents and Chemotherapy 36 (8):1688.

Boyer et al. (2001) YADD mutants of human immunodeficiency virus type 1 and Moloney murine leukemia virus reverse transcriptase are resistant to lamivudine triphosphate (3TCTP) in vitro. J. Virol. 75:6321.

Feng et al. (1999) Mechanistic studies comparing the incorporation of (+) and (-) isomers of 3TCTP by HIV-1 reverse transcriptase. Biochemistry 38:55.

Kewn et al. (2002) Development of enzymatic assays for quantification of intracellular lamivudine and carbovir triphosphate levels in peripheral blood mononuclear cells from human immunodeficiency virus-infected patients. Antimicrob. Agents Chemother. 46:135.