AP4G

For general laboratory use.

Envio: shipped on gel packs

Condições de armazenamento: store at -20 °C
Short term exposure (up to 1 week cumulative) to ambient temperature possible.

Validade: 12 months after date of delivery

Fórmula molecular: C20H28N10O20P4 (free acid)

Peso molecular: 852.39 g/mol (free acid)

CAS#: 10527-46-5

Pureza: ≥ 95 % (HPLC)

Forma: solution in water

Concentração: 10 mM - 11 mM

pH: 7.5 ±0.5

Propriedades espectroscópicas: λmax 259 nm, ε 27.0 L mmol-1 cm-1 (Tris-HCl pH 7.5)

Formulários:
Formation by T4 RNA ligase^[1]
Formation by Pfu DNA ligase^[2]
Acceptor substrate for T4 RNA ligase^[3]

Referências selecionadas:
[1] Sillero et al. (2006) Synthesis of bisphosphonate derivatives of ATP by T4 RNA ligase. FEBS Lett. 580 (24):5723.

[2] Günther et al. (2002) Thermostable Pyrococcus furiosus DNA ligase catalyzes the synthesis of (di)nucleoside polyphosphates. Extremophiles. 6 (1):45.

[3] Atencia et al. (2000) Several dinucleoside polyphosphates are acceptor substrates in the T4 RNA ligase catalyzed reaction. Eur. J. Biochem. 267 (6):1707.

Winward et al. (2010) Oxidation of the diphosphoinositol polyphosphate phosphohydrolase-like Nudix hydrolase Aps from Drosophila melanogaster induces thermolability--A possible regulatory switch≥ Int. J. Biochem. Cell Biol. 42 (7):1174.

Safrany et al. (2007) Characterisation of a bis (5'-nucleosyl)-tetraphosphatase (asymmetrical) from Drosophila melanogaster. Int. J. Biochem. Cell Biol. 39 (5):943.

Leslie et al. (2002) Cloning and characterisation of hAps1 and hAps2, human diadenosine polyphosphate-metabolising Nudix hydrolases. BMC Biochemistry 3:20.

Ortiz et al. (1993) Specific synthesis of adenosine (5')tetraphospho (5')nucleoside and adenosine- (5')oligophospho (5')adenosine (n-greaterthan- 4) catalyzed by firefly luciverase. Eur. J. Biochem. 212 (1):263.

Palfi et al. (1991) Alterations in the accumulation of adenylylated nucleotides in heavy-metal-ion-stressed and heat-stressed Synechococcus sp strain pcc-6301, a cyanobacterium, in light and dark. Biochem. J. 276:487.

Brevet et al. (1991) Isolation and characterization of a dinucleoside triphosphatase from Saccharomyces-cerevisiae. J. Bacteriol. 173 (17):5275.

Sillero et al. (1991) Synthesis of dinucleoside polyphosphates catalyzed by firefly luciferase. Eur. J. Biochem. 202 (2):507.

Avila et al. (1991) A paradoxical increase of a metabolite upon increased expression of its catabolic enzyme - the case of diadenosine tetraphosphate (AP4A) and AP4A phosphorylase-I in Saccharomyces-cerevisiae. J. Bacteriol. 173 (24):7875.

Barnes et al. (1984) Measurement of AP4A and AP4G during the cell-cycle of Physarumpolycephalum. H-S Z. Physiol. Chem. 365 (6):608.

Bochner et al. (1984) AppppA and related adenylylated nucleotides are synthesized as a consequence of oxidation stress. Cell 37 (1):225.

Garrison et al. (1984) Assay of adenosine 5'-P1-tetraphospho-P4-5'''-adenosine and adenosine 5'-P1-tetraphospho-P4-5'''-guanosine in Physarum-polycephalum and other eukaryotes - an isocratic high-pressure liquid-chromatography method. Biochem. J. 217 (3):805.