5-Methyl-CTP
m5CTP
5-Methylcytidine-5'-triphosphate, Sodium salt
5-Me-CTP
| Catálogo Nº | Apresentação | Preço (R$) | Comprar |
|---|---|---|---|
| NU-1138S | 10 μl (100 mM) | Sob demanda | Adicionar ao Carrinho |
| NU-1138L | 5 x 10 μl (100 mM) | Sob demanda | Adicionar ao Carrinho |
For general laboratory use.
Envio: shipped on gel packs
Condições de armazenamento: store at -20 °C
Short term exposure (up to 1 week cumulative) to ambient temperature possible.
Validade: 12 months after date of delivery
Fórmula molecular: C10H18N3O14P3 (free acid)
Peso molecular: 497.18 g/mol (free acid)
CAS#: 327174-86-7 (acid)
Pureza: ≥ 95 % (HPLC)
Forma: solution in water
Concentração: 100 mM - 110 mM
pH: 7.5 ±0.5
Propriedades espectroscópicas: λmax 277 nm, ε 9.0 L mmol-1 cm-1 (Tris-HCl pH 7.5)
Formulários:
Functional influence of modified bases[1-5]
Incorporation by PCR[3]
Produtos relacionados: HighYield T7 RNA Synthesis Kit, #RNT-101
Referências selecionadas:
[1] Adams et al. (2014) 185 CELLULAR VIABILITY AND EXPRESSION OF GREEN FLUORESCENT PROTEIN IN BOVINE FETAL FIBROBLASTS FOLLOWING TRANSFECTION OF SYNTHETIC mRNA INCLUDING MODIFIED BASES. Reprod. Fertil. Dev. 26:207.
[2] Tchigvintsev et al. (2013) Biochemical and Structural Studies of Conserved Maf Proteins Revealed Nucleotide Pyrophosphatases with a Preference for Modified Nucleotides. Chem. Biol. Oxford U.K. 20:1386.
[3] Wong et al. (1997) A novel method for producing partial restriction digestion of DNA fragments by PCR with 5-methyl-CTP. Nucleic Acids Res. 25:4169.
[4] Kormann et al. (2011) Expression of therapeutic proteins after delivery of chemically modified mRNA in mice. Nature Biotechnology 29:154.
[5] Warren et al. (2010) Highly efficient reprogramming to pluripotency and directed differentiation of human cells with synthetic modified mRNA. Cell Stem Cell 7 (5):618.