5-Methyl-dCTP

For general laboratory use.

Envio: shipped on gel packs

Condições de armazenamento: store at -20 °C
Short term exposure (up to 1 week cumulative) to ambient temperature possible.

Validade: 12 months after date of delivery

Fórmula molecular: C10H18N3O13P3 (free acid)

Peso molecular: 481.18 g/mol (free acid)

CAS#: 22003-12-9

Pureza: ≥ 95 % (HPLC)

Forma: solution in water

Concentração: 100 mM - 110 mM

pH: 7.5 ±0.5

Propriedades espectroscópicas: λmax 277 nm, ε 9.0 L mmol-1 cm-1 (Tris-HCl pH 7.5)

Formulários:
Incorporation into DNA by
- PCR with Taq polymerase ^{in-house data, [1-2]}

Descrição:
5-methylated DNA probes can be used as methylation reference fragment [1-2] or for pull-down of 5-hmC binding proteins from cellular lysate[3].

Referências selecionadas:
[1] Szwagierczak et al. (2011) Characterization of PvuRts1I endonuclease as a tool to investigate genomic 5-hydroxymethylcytosine. Nucl. Acids Res. 39(12):5149.
[2] Szwagierczak et al. (2010) Sensitive enzymatic quantification of 5-hydroxymethylcytosine in genomic DNA. Nucl. Acids Res. 38(19):e181.
[3] Lafaye et al. (2014) DNA binding of the p21 repressor ZBTB2 is inhibited by cytosine hydroxymethylation. Biochem. Biophys. Res. Commun. 446:341.


Kaito et al. (2001) Activation of the maternally preset program of apoptosis by microinjection of 5-aza-2'-deoxycytidine and 5-methyl-2'-deoxycytidine-5'-triphosphate in Xenopus laevis embryos. Dev Growth Differ. 43 (4):383.

Wu et al. (2007) Crystal structure of RS21-C6, involved in nucleoside triphosphate pyrophosphohydrolysis. J. Mol. Biol. 367:1405.

Dezhurov et al. (2005) A new highly efficient photoreactive analogue of dCTP. Synthesis, characterization, and application in photoaffinity modification of DNA binding proteins. Bioconjug. Chem. 16 (1):215.

Song et al. (2015) Human dCTP pyrophosphatase 1 promotes breast cancer cell growth and stemness through the modulation on 5-methyl-dCTP metabolism and global hypomethylation. Oncogenesis 4: e159.

Frauer et al. (2009) A versatile non-radioactive assay for DNA methyltransferase activity and DNA binding. Nucleic Acids Research 37 (3):e22.

Zeschnigk et al. (2009) Massive parallel bisulfite sequencing of CG-rich DNA fragments reveals that methylation of many X-chromosomal CpG islands in female blood DNA is incomplete. Human Molecular Genetics 18 (8):1439.

Holliday et al. (2002) DNA methylation and epigenetic inheritance. Methods. 27 (2):179.

Asamizu et al. (1999) A large scale structural analysis of cDNAs in a unicellular green alga, Chlamydomonas reinhardtii. I. Generation of 3433 non-redundant expressed sequence tags. DNA Res. 6 (6):369.

Ito et al. (1998) Systematic method to obtain novel genes that are regulated by mi transcription factor: impaired expression of granzyme B and tryptophan hydroxylase in mi/mi cultured mast cells. Blood 91 (9):3210.

Lefaucheur et al. (1998) Evidence for three adult fast myosin heavy chain isoforms in type II skeletal muscle fibers in pigs. J. Anim. Sci. 76 (6):1584.

Wong et al. (1997) A novel method for producing partial restriction digestion of DNA fragments by PCR with 5-methyl-CTP. Nucleic Acids Res. 25 (20):4169.

Chen et al. (1993) Direct induction of DNA hypermethylation in sea urchin embryos by microinjection of 5-methyl dCTP stimulates early histone gene expression and leads to developmental arrest. Dev Biol. 155 (1):75.
[3] Lafaye et al. (2014) DNA binding of the p21 repressor ZBTB2 is inhibited by cytosine hydroxymethylation. Biochem. Biophys. Res. Commun. 446:341.

Nelson et al. (1993) Restriction endonuclease cleavage of 5-methyl-deoxycytosine hemimethylated DNA at high enzyme-to-substrate ratios. Nucl. Acid. Res. 21 (3):681.