5-Vinyl-uridine (5-VU)

For general laboratory use.

Envio: shipped at ambient temperature

Condições de armazenamento: store at -20 °C
Short term exposure (up to 1 week cumulative) to ambient temperature possible.

Validade: 12 months after date of delivery

Fórmula molecular: C11H14N2O6

Peso molecular: 270.24 g/mol

CAS#: 55520-64-4

Pureza: ≥ 98 % (HPLC)

Forma: solid

Solubilidade: DMSO, methanol

Propriedades espectroscópicas: λmax 288 nm, ε 8.5 L mmol-1 cm-1 (Methanol)

Formulários:
RNA synthesis monitoring (potentially)

Descrição:
5-VU (5-Vinyl-uridine) can potentially be used as a replacement for BrU (5-Bromo-uridine) or the copper-catalyst requiring 5-EU (5-Ethynyl-uridine) to measure de novo RNA synthesis in proliferating cells. 5-VU is cell permeable and might incorporate into nascent RNA instead of its natural analog uridine. This assumption is based on the previously demonstrated suitability of 5-VdU (5-Vinyl-2'-deoxyuridine) for DNA synthesis monitoring[1].

The resulting vinyl-functionalized RNA could subsequently be detected via Cu(I)-free Alkene-Tetrazine Ligation that offers the choice to introduce a Biotin group (via Tetrazines of Biotin) for subsequent purification tasks or a fluorescent group (via Tetrazines of fluorescent dyes) for subsequent microscopic imaging.

Produtos relacionados: 5-Ethynyl-uridine (5-EU), #CLK-N002

Referências selecionadas:
[1] Rieder et al. (2014) Alkene-Tetrazine Ligation for Imaging Cellular DNA. Angew. Chem. Int. Ed. 53:9168.

Liu et al. (2019) A Nucleoside Derivative 5-Vinyluridine (VrU) for Imaging RNA in Cells and Animals. Bioconjug. Chem. 30 (11):2958.