Figure 1: A) Workflow of RNA synthesis monitoring with 5-EU using copper-catalyzed Alkyne-Azide Ligation. B) Schematic overview of RNA labeling using vinyl-functionalized nucleosides and imaging through bioorthogonal IEDDA reaction with Pyrimidyl tetrazines.

Selected References

[1] Jao et al. (2008) Exploring RNA transcription and turnover in vivo by using click chemistry. Proc. Natl. Acad. Sci. USA 105 (41):15779.
[2] Darzynkiewicz et al. (2011) Cytometry of DNA Replication and RNA Synthesis: Historical Perspective and Recent Advances Based on "Click Chemistry". Cytometry A 79A:328.
[3] Liu et al. (2019) A Nucleoside Derivative 5‑Vinyluridine (VrU) for Imaging RNA in Cells and Animals. Bioconjugate Chem. 30:2958.
[4] Kubota et al. (2019) Expanding the Scope of RNA Metabolic Labeling with Vinyl Nucleosides and Inverse Electron-Demand Diels−Alder Chemistry. ACS Chem. Biol. 14:1698.