cDNA Synthesis Kit with RNAse Inhibitor
Kit de síntese de cDNA com inibidor de RNase
| Catálogo Nº | Apresentação | Preço (R$) | Comprar |
|---|---|---|---|
| PRT-110XS | 20 reações | 322,91 | Adicionar ao Carrinho |
| PRT-110S | 100 reações | 1.291,62 | Adicionar ao Carrinho |
| PRT-110L | 200 reações | 2.260,85 | Adicionar ao Carrinho |
| PRT-110XL | 500 reações | 5.597,02 | Adicionar ao Carrinho |
Descrição:
SCRIPT cDNA Synthesis Kit contains all reagents required for first strand cDNA synthesis in one box combining simple handling with high flexibility. The premium quality Reverse Transcriptase, ultrapure dNTPs and an optimized reaction buffer ensure superior results with highest reproducibility. The kit is optimized for high efficiency in a broad range of primer-template combinations. SCRIPT Reverse Transcriptase is a genetically engineered version of M-MLV Reverse Transcriptase (M-MLV RT) with eliminated RNase H activity and increased thermal stability. The enzyme is a RNA-directed DNA polymerase that synthesizes a complementary DNA strand initiating from a primer using single-stranded RNA or DNA as template. Its enhanced thermal stability in combination with the deactivated RNase H activity results in an increased specificity, higher cDNA yield and an improved efficiency for full length cDNA synthesis compared with standard M-MLV RT. The enzyme is recommended for synthesis of cDNA from 100 bp up to 10 kb length.
Activity and stability has been tested in first strand cDNA synthesis.
Contente:
SCRIPT Reverse Transcriptase #PRT-102 (blue cap)
200 units/μl in 20 mM Tris-HCl, 100 mM NaCl, 1 mM EDTA, 5 mM DTT and stabilizers
SCRIPT Reverse Transcriptase buffer 5x (red cap)
250 mM Tris-HCl (pH 8.3), 500 mM KCl, 30 mM MgCl2, 25 Mm DTT
DTT stock solution (purple cap)
100 mM DTT
dNTP Mix #NUC-100 (white cap)
10 mM each dNTP
Random Hexamers (white cap)
100 μM
Rnase Inhibitor #PCK-122 (blue cap)
40 units/μl
RNAse-free water (white cap)