FTaseGST
Protein farnesyltransferase, α- and β-subunit
rat, recombinant, E. coli
| Catálogo Nº | Apresentação | Preço (R$) | Comprar / Observação |
|---|---|---|---|
| PR-361 | 50 μg | Sob demanda | Adicionar ao Carrinho |
For in vitro use only!
Envio: shipped on dry ice
Condições de armazenamento: store at -80 °C
avoid freeze/thaw cycles
Validade: 12 months
Peso molecular: α: 70 kDa, β: 35 kDa
Número de acesso: Q04631 / Q02293
Número de acesso: Q04631 / Q02293
Pureza: > 90 % (SDS-PAGE)
Forma: liquid (Supplied in 100 mM HEPES pH 7.2, 100 mM NaCl and 5 mM DTT)
Atividade: 1 pmol of FTase will transfer 1 pmol of farnesyl to H-Ras in 15 min at 37°C.
Descrição:
FTase catalyzes the transfer of the farnesyl group from farnesyl diphosphate to proteins containing a C-terminal CaaX motif, where 'C' is a conserved cysteine that is the site of farnesyl modification, 'a' is usually an aliphatic amino acid, and 'X' is methionine, serine, glutamine, or alanine. The GST-Tag facilitates the protein`s application in typical GST pull-down assays.
Referências selecionadas:
Lackner et al. (2005) Chemical genetics indentifies Rab geranylgeranyl transferase as an apoptotic target of farnesyl transferase inhibitors. Cancer Cell. 7:325.
Zimmerman et al. (1998) High-level expression of rat farnesyl:proteintransferase in Escherichia coli as a translationally coupled heterodimer. Protein Express. Purif. 14:395.