GGTase-IGST

For general laboratory use.

Envio: shipped on dry ice

Condições de armazenamento: store at -80 °C
avoid freeze/thaw cycles

Validade: 12 months

Peso molecular: α: 44 kDa, β: 38 kDa

Número de acesso: NM_031082

Número de acesso: NM_031082

Pureza: > 90 % (SDS-PAGE)

Forma: liquid (Supplied in 25 mM HEPES pH 7.2, 25 mM NaCl and 5 mM DTT)

Atividade: 1 pmol of GGTase-I will transfer 5 pmol of Farnesyl to RhoA in 15 min at 37°C.

Descrição:
GGTase-I (Geranylgeranyltransferase-I) catalyzes the transfer of the farnesyl and geranylgeranyl groups from farnesyl and geranylgeranyl-diphosphate to proteins containing a C-terminal CaaX motif, where 'C' is a conserved cysteine that is the site of farnesyl modification, 'a' is usually an aliphatic amino acid, and 'X' is leucine or phenylalanine. Farnesyltransferase (FT) and GGTase-I are closely related, sharing a common α subunit and 30% identity in their β subunits. The GST-Tag facilitates the protein`s application in typical GST pull-down assays.

Referências selecionadas:
Lackner et al. (2005) Chemical genetics indentifies Rab geranylgeranyl transferase as an apoptotic target of farnesyl transferase inhibitors. Cancer Cell. 7:325. Yokoyama et al. (1993) Purification of a mammalian protein geranylgeranyltransferase. Formation and catalytic properties of an enzyme-geranylgeranyl pyrophosphate complex. J. Biol. Chem. 268:4055.