AF6-RBDGST (residues 1-141)

For general laboratory use.

Envio: shipped on gel packs

Condições de armazenamento: store at -20 °C
avoid freeze/thaw cycles

Validade: 12 months

Número de acesso: NM_005936

Número de acesso: NM_005936

Pureza: > 90 % (SDS-PAGE)

Forma: liquid (Supplied in 25 mM HEPES pH 7.5, 50 mM NaCl, 1 mM EDTA and 50% glycerol)

Descrição:
The amino terminus of Af6 was identified as the Rasbinding site. Additionally to the Ras-binding Af6 also binds Rap. ZO-1, a protein involved in the formation of tight junctions, also binds to Af6 close to the amino terminus thereby competing with Ras binding. These data suggest a participation of Af6 in the regulation of cell-cell contacts via a Ras-modulated interaction with ZO-1. In contrast to the well characterized effectors Raf, RalGEF, and PI(3)kinase, Af6 is a protein with no enzymatic function. It seems to serve as a scaffolding component for protein complexes. This would represent a new type of Ras effector because Ras binding in this case does not lead to the activation of the enzymatic activity of the effector.

Referências selecionadas:
Linnemann et al. (1999) Thermodynamic and Kinetic Characterization of the Interaction between the Ras Binding Domain of AF6 and Members of the Ras Subfamily. J. Biol. Chem. 274:13556. Steiner et al. (2000) Sequence-specific resonance assignment of the Ras-binding domain of AF6. J. Biomol. NMR. 18:73.