Proteínas e Enzimas Restrição/Modificadoras

RhotekinRBDGST

Rhotekin Ras Binding Domain, from the Japanese

human, recombinant, E. coli

Catálogo Nº	Apresentação	Preço (R$)	Comprar / Observação
PR-367	50 μg	Sob demanda	Adicionar ao Carrinho

For general laboratory use.

Envio: shipped on dry ice

Condições de armazenamento: store at -80 °C
avoid freeze/thaw cycles

Validade: 12 months

Número de acesso: NM_033046

Pureza: > 90 % (SDS-PAGE)

Forma: liquid (Supplied in 50 mM Tris-HCl pH 7.5, 100 mM NaCl and 2 mM DTE)

Descrição:
Rhotekin (from the Japanese teki, meaning target) is an effector of the Rho-GTPases that was previously identified in mice. GST-RhotekinRBD is a glutathione S-transferase (GST)-fusion protein of the Rho-binding domain (RBD) of Rhotekin. The N-terminal peptide of Rhotekin contains the amino acids 1-89 comprising the Rho-binding domain between amino acids 7 and 89. The GST-Tag facilitates the protein`s application in typical GST pull down assays.

Referências selecionadas:
Reynaud et al. (2000) The PDZ protein TIP-1 interacts with the Rho effector Rhotekin and is involved in Rho signaling to the serum response element. J. Biol. Chem. 275:33962. Reid et al. (1996) Rhotekin, a new putative target for Rho bearing homology to the serine/threonine kinase, PKN, and rhodophilin in the Rho-binding domain. J. Biol. Chem. 271:13556.