Rab3A^GST-His

For in vitro use only!

Envio: shipped on dry ice

Condições de armazenamento: store at -80 °C
avoid freeze/thaw cycles

Validade: 12 months

Número de acesso: NP_037150

Número de acesso: NP_037150

Pureza: > 90 % (SDS-PAGE)

Forma: liquid (Supplied in 50 mM Tris-HCl pH 8.0, 100 mM NaCl, 10 mM MgCl₂, 10 μM GDP and 2 mM mercaptoethanol)

Atividade: 100 pmol of protein can bind > 80 pmol of GDP.

Descrição:
Rab3A is a small GTPase that belongs to the Ras superfamily. Rab proteins play an important role in various aspects of membrane traffic, including cargo selection, vesicle budding, vesicle motility, tethering, docking, and fusion. Rab3A is involved in the process of Ca2+-dependent neurotransmitter release. GTP-Rab3A binds effector molecules, Rabphilin-3 and Rim, localized at synaptic vesicles and the active zone, respectively. These complexes facilitate translocation and docking of the synaptic vesicles to the active zone. The GST-Tag facilitates the protein`s application in typical GST pull-down assays.

Referências selecionadas:
Stenmark et al. (2001) The Rab GTPase family. Genome Biol. 2:30071.
Yamaguchi et al. (2002) A GDP/GTP exchange protein for the Rab3 small G protein family up-regulates a postdocking step of synaptic exocytosis in central synapses. Proc. Natl. Acad. Sci. USA. 99:14536.