FTaseW102T_Y154TGST
Protein farnesyltransferase mutant, α- and β-subunit
rat, recombinant, E. coli
| Catálogo Nº | Apresentação | Preço (R$) | Comprar / Observação |
|---|---|---|---|
| PR-952 | 20 μg | Sob demanda | Adicionar ao Carrinho |
For general laboratory use.
Envio: shipped on dry ice
Condições de armazenamento: store at -80 °C
avoid freeze/thaw cycles
Validade: 12 months
Peso molecular: GST-α: 73 kDa, β: 48 kDa
Pureza: > 90 % (SDS-PAGE)
Forma: liquid (Supplied in 25 mM HEPES pH 7.2, 40 mM NaCl and 2 mM DTT)
Atividade: 1 pmol of FTase double mutant will transfer 0.4 pmol of BGPP to H-Ras in 15 min at 37°C.
Descrição:
FTase is one of the three mammalian protein prenyltransferases. The double mutant of FTase was engineered to efficiently transfer biotin-geranylpyrophosphate (BGPP, Cat.# LI-015) onto its native protein substrates. This approach allows the detection of femtomolar amounts of prenylatable proteins in eukaryotic cells and tissues as well as effective inhibitor screening.
Referências selecionadas:
Hougland et al. (2009) Getting a handle on protein prenylation. Nat. Chem. Biol. 5 (4):197. Nguyen et al. (2009) Analysis of the eukaryotic prenylome by isoprenoid affinity tagging. Nat. Chem. Biol. 5 (4):227.