NBD-FPP

For general laboratory use.

Envio: shipped on gel packs

Condições de armazenamento: store at -20 °C
avoid freeze/thaw cycles, store dark

Validade: 12 months

Fórmula molecular: C21H30N4O10P2

Peso molecular: 560.43 g/mol

Pureza: ≥ 95 % (HPLC)

Forma: liquid (Supplied as 1 mM solution in 25 mM (NH4)2CO3)

Concentração: 1 mM

Propriedades espectroscópicas: λexc 480 nm, λem 530 nm, ε 11.0 L mmol-1 cm-1 (pH 7.5)

Descrição:
NBD-FPP is a fluorescent analog of farnesyl pyrophosphate (FPP). It serves as lipid donor for geranylgeranyltransferases (RabGGTase, GGTase-I). NBD-FPP changes its fluorescence upon binding to protein substrates and allows for efficient fluorescence based GGTase activity assays and inhibitor screening.

Referências selecionadas:
Nguyen et al. (2009) Analysis of the eukaryotic prenylome by isoprenoid affinity tagging. Nature Chemical Biology. 5 (4):227. Wu et al. (2007) Synthesis of a fluorescent analogue of geranylgeranyl pyrophosphate and its use in a high-throughput fluorometric assay for Rab geranylgeranyltransferase. Nat. Protoc. 2 (11):2704. Dursina et al. (2006) Identification and specificity profiling of protein prenyltransferase inhibitors using new fluorescent phosphoisoprenoids. J. Am. Chem. Soc. 128 (9):2822.