SuperTaq Pol
DNA Polimerase com tampão corante de rastreio
| Catálogo Nº | Apresentação | Preço (R$) | Comprar |
|---|---|---|---|
| POL-110XS | 250 U | 225,16 | Adicionar ao Carrinho |
| POL-110S | 500 U | 414,76 | Adicionar ao Carrinho |
| POL-110L | 2,50 U | 1.806,01 | Adicionar ao Carrinho |
Definição de unidade: One unit is defined as the amount of the enzyme required to catalyze the incorporation of 10 nmoles of dNTPs into an acid- insoluble form in 30 minutes at 70 °C.
Envio: Shipped on blue ice
Condições de armazenamento: Store at -20 °C
Avoid freeze/thaw cycles
Validade: 36 months
Concentração: 5 units/uL
Descrição:
SuperTaq Pol is recommended for use in routine PCR reactions. The buffer system is optimized for high efficiency and gives superior amplification results in a broad range of reaction conditions with most primer-template pairs. The buffer system facilitates the incorporation of labeled or modified nucleotides into DNA. Note that the ammonium based buffer contains detergents and may interfere with automated pipetting systems.
The enzyme replicates DNA at 72 °C. It catalyzes the polymerization of nucleotides into duplex DNA in 5'-→3' direction in the presence of magnesium. It also possesses a 5'→3' polymerization-dependent exonuclease replacement activity but lacks a 3'→5' exonuclease activity.
Contente:
SuperTaq Pol (blue cap)
5 units/μl SuperTaq Pol in Tris-HCl pH 8.0 (25°C), KCl, EDTA, DTT, 50% (v/v) Glycerol, and stabilizers.
SuperTaq Colorless Reaction Buffer (red cap) - 5x conc.
Proprietary formulation supplied at pH 8,5. DOES NOT CONTAIN MgCl2.
SuperTaq Green Reaction Buffer (red cap) - 5x conc.
Is supplemented with tracking dyes for direct loading of PCR products on gels. DOES NOT CONTAIN MgCl2.
MgCl2 Stock Solution (yellow cap)
25 mM MgCl2.