5-Bromo-dUTP

For general laboratory use.

Envio: shipped on gel packs

Condições de armazenamento: store at -20 °C
Short term exposure (up to 1 week cumulative) to ambient temperature possible.

Validade: 12 months after date of delivery

Fórmula molecular: C9H14N2O14P3Br (free acid)

Peso molecular: 547.04 g/mol (free acid)

CAS#: 102212-99-7

Pureza: ≥ 95 % (HPLC)

Forma: solution in water

Concentração: 10 mM - 11 mM

pH: 7.5 ±0.5

Propriedades espectroscópicas: λmax 278 nm, ε 9.7 L mmol-1 cm-1 (Tris-HCl pH 7.5)

Referências selecionadas:
Ma et al. (2008) The mutagenic properties of BrdUTP in a random mutagenesis process. Mol. Biol. Rep. 35:663.

Pal et al. (2002) Strong natural pausing by RNA polymerase II within 10 bases of transcription start may result in repeated slippage and reextension of the nascent RNA. Mol. Cell Biol. 22 (1):30.

Srinivasan et al. (2002) Characterization of RNA polymerase III transcription factor TFIIIC from the mulberry silkworm, Bombyx mori. Eur. J. Biochem. 26986):1780.

Mote et al. (1998) Recognition of a human arrest site is conserved between RNA polymerase II and prokaryotic RNA polymerases. J. Biol. Chem. 273 (27):16843.

Ekstrand (1996) A sensitive assay for the quantification of reverse transcriptase activity based on the use of carrier-bound template and non-radioactive-product detection, with special reference to human-immunodeficiency-virus isolation. Biotechnol Appl. Biochem. 23-2:95.

Gorczyca (1996) Laser scanning cytometer (LSC) analysis of fraction of labelled mitoses (FLM). Cell Prolif. 29 (10):539.

Li et al. (1995) Labelling DNA strand breaks with BrdUTP. Detection of apoptosis and cell proliferation. Cell Prolif. 28 (11):571.

Ji et al. (1991) T4-phage ribonucleotide reductase - allosteric regulation invivo by thymidine triphosphate. J. Biol. Chem. 266 (25):16289.