C8-Alkyne-dCTP

5-(Octa-1,7-diynyl)-2'-deoxycytidine 5'-triphosphate, Sodium salt

Catálogo Nº	Apresentação	Preço (R$)	Comprar
CLK-T06-S	5 μl (100 mM)	Sob demanda	Adicionar ao Carrinho
CLK-T06-L	5 x 5 μl (100 mM)	Sob demanda	Adicionar ao Carrinho
CLK-T06-XL	50 μl (100 mM)	Sob demanda	Adicionar ao Carrinho

For general laboratory use.

Envio: shipped on gel packs

Condições de armazenamento: store at -20 °C
Short term exposure (up to 1 week cumulative) to ambient temperature possible.

Validade: 12 months after date of delivery

Fórmula molecular: C17H24N3O13P3 (free acid)

Peso molecular: 571.31 g/mol (free acid)

Pureza: ≥ 95 % (HPLC)

Forma: solution in water

Concentração: 100 mM

pH: 7,5

Propriedades espectroscópicas: ε260 nm 7.3 L mmol-1 cm-1 (in water), ε292 nm 9.5 L mmol-1 cm-1 (in water)

Formulários:
Incorporation into DNA by PCR with family B polymerases (Pwo, Deep Vent exo^- or KOD XL)^[1,2]
Incorporation into DNA by Primer Extension with family A polymerase Taq and family B polymerases (Pwo, Deep Vent exo^- or KOD XL)^[1]
Incorporation into DNA by 3'-End Labeling with Terminal deoxynucleotidyl Transferase(TdT)^[3]
The resulting ethynyl-functionalized DNA can subsequently be processed via Cu(I)-catalyzed click chemistry that offers the choice

to introduce a Biotin group (via Azides of Biotin) for subsequent purification tasks
to introduce fluorescent group (via Azides of fluorescent dyes) for subsequent microscopic imaging
to crosslink the DNA to Azide-functionalized biomolecules e.g.proteins

Presolski et al.^[4] and Hong et al.^[5] provide a general protocol for Cu(I)-catalyzed click chemistry reactions that may be used as a starting point for the set up and optimization of individual assays.

Produtos relacionados:

Copper (II)-Sulphate (CuSO4), #CLK-MI004
Tris(3-hydroxypropyltriazolylmethyl)amine (THPTA), #CLK-1010
Sodium Ascorbate (Na-Ascorbate), #CLK-MI005

Referências selecionadas:
[1] Gierlich et al. (2007) Synthesis of Highly Modified DNA by a Combination of PCR with Alkyne-Bearing Triphosphates and Click Chemistry. Chem. Eur. J. 13:9486.

[2] Burley et al. (2006) Directed DNA Metallization. J. Am. Chem. Soc. 128 (5):1398.

[3] Sorensen et al. (2013) Enzymatic Ligation of Large Biomolecules to DNA. ACSnano 7 (9):8098.

[4] Presolski et al. (2011) Copper-Catalyzed Azide-Alkyne Click Chemistry for Bioconjugation. Current Protocols in Chemical Biology 3:153.

[5] Hong et al. (2011) Analysis and Optimization of Copper-Catalyzed Azide-Alkyne Cycloaddition for Bioconjugation. Angew. Chem. Int. Ed. 48:9879.