UTPαS

Uridine-5'-(α-thio)-triphosphate, Sodium salt; (Mixture of Rp and Sp isomers)

(1 : 1 Mixture of Rp and Sp isomers)

Catálogo Nº Apresentação Preço (R$) Comprar
NU-411S 25 μl (100 mM)Sob demanda Adicionar ao Carrinho
NU-411L 5 x 25 μl (100 mM)Sob demanda Adicionar ao Carrinho
Structural formula of UTPαS (Uridine-5'-(α-thio)-triphosphate, Sodium salt; (Mixture of Rp and Sp isomers))
Structural formula of UTPαS

For general laboratory use.

Envio: shipped on gel packs

Condições de armazenamento: store at -20 °C
Short term exposure (up to 1 week cumulative) to ambient temperature possible.

Validade: 12 months after date of delivery

Fórmula molecular: C9H15N2O14P3S (free acid)

Peso molecular: 500.20 g/mol (free acid)

CAS#: 71214-29-4

Pureza: ≥ 95 % (HPLC)

Forma: solution in water

Concentração: 100 mM - 110 mM

pH: 7.5 ±0.5

Propriedades espectroscópicas: λmax 262 nm, ε 10.0 L mmol-1 cm-1 (Tris-HCl pH 7.5)

Referências selecionadas:
Oyelere et al. (2002) pKa perturbation in genomic hepatitis delta virus ribozyme catalysis evidenced by nucleotide analogue interference mapping. Biochemistry-US 41 (11):3667.

Basu et al. (2001) Biochemical detection of monovalent metal ion binding sites within RNA. Methods 23 (3):264.

Oyelere et al. (2000) Biochemical detection of cytidine protonation within RNA. J. Am. Chem. Soc. 122 (42):10259.

Vortler et al. (2000) Phosphorothioate modification of RNA for stereochemical and interference analyses. Method. Enzymol. 317:74.

Ryder et al. (2000) Chemical probing of RNA by nucleotide analog interference mapping. Method. Enzymol. 317:92.

Burgess et al. (2000) Syntheses of nucleoside triphosphates. Chem. Rev. 100 (6):2047.

Ryder et al. (1999) Nucleotide analog interference mapping. Methods 18 (1):38.

Strobel (1999) A chemogenetic approach to RNA function/structure analysis. Curr. Opin. Struc. Biol. 9 (3):346.

Boudvillain et al. (1998) Defining functional groups, core structural features and inter-domain tertiary contacts essential for group II intron self-splicing: a NAIM analysis. EMBO J. 17 (23):7091.

Basu et al. (1998) A specific monovalent metal ion integral to the AA platform of the RNA tetraloop receptor. Nat. Struct. Biol. 5 (11):986.

Ortoleva-Donnelly et al. (1998) Identifying RNA minor groove tertiary contacts by nucleotide analogue interference mapping with N-2-methylguanosine. Biochemistry-US 37 (37):12933.

Arabshahi et al. (1994) A simplified procedure for synthesizing nucleoside 1-thiotriphosphates - dATP (alpha)S, dGTP (alpha)S, UTP-alpha-S, and dTTP (alpha)S. Biochem. Biophys. Res. Co. 204 (1):150.