5-Ethynyl-UTP (5-EUTP)

5-Ethynyl-uridine-5'-triphosphate, Sodium salt

Catálogo Nº	Apresentação	Preço (R$)	Comprar
CLK-T08-S	5 μl (100 mM)	Sob demanda	Adicionar ao Carrinho
CLK-T08-L	5 x 5 μl (100 mM)	Sob demanda	Adicionar ao Carrinho
CLK-T08-XL	50 μl (100 mM)	Sob demanda	Adicionar ao Carrinho

For general laboratory use.

Envio: shipped on gel packs

Condições de armazenamento: store at -20 °C
Short term exposure (up to 1 week cumulative) to ambient temperature possible.

Validade: 12 months after date of delivery

Fórmula molecular: C11H15N2O15P3 (free acid)

Peso molecular: 508.16 g/mol (free acid)

Pureza: ≥ 95 % (HPLC)

Forma: solution in water

Concentração: 100 mM - 110 mM

pH: 7.5 ±0.5

Propriedades espectroscópicas: λmax 288 nm, ε 12.0 L mmol-1 cm-1 (Tris-HCl pH 7.5)

Formulários:
Incorporation into RNA by T7 RNA polymerase-mediated in vitro transcription.
The resulting alkyne-functionalized RNA can subsequently be processed via Cu(I)-catalyzed (azide-alkyne) click chemistry that offers the choice

to introduce a Biotin group for subsequent purification tasks (via Azides of Biotin)
to introduce fluorescent group for subsequent microscopic imaging (via Azides of fluorescent dyes)
to crosslink the RNA to azide-functionalized biomolecules e.g.proteins

Presolski et al.^[1] and Hong et al.^[2] provide a general protocol for Cu(I)-catalyzed click chemistry reactions that may be used as a starting point for the set up and optimization of individual assays.

Produtos relacionados:

Copper (II)-Sulphate (CuSO4), #CLK-MI004
Tris(3-hydroxypropyltriazolylmethyl)amine (THPTA), #CLK-1010
Sodium Ascorbate (Na-Ascorbate), #CLK-MI005

Referências selecionadas:
[1] Presolski et al. (2011) Copper-Catalyzed Azide-Alkyne Click Chemistry for Bioconjugation. Current Protocols in Chemical Biology 3:153.

[2] Hong et al. (2011) Analysis and Optimization of Copper-Catalyzed Azide-Alkyne Cycloaddition for Bioconjugation. Angew. Chem. Int. Ed. 48:9879.