5-Ethynyl-dUTP (5-EdUTP)

5-Ethynyl-2'-deoxyuridine 5'-triphosphate, Sodium salt

Catálogo Nº	Apresentação	Preço (R$)	Comprar
CLK-T07-S	5 μl (100 mM)	Sob demanda	Adicionar ao Carrinho
CLK-T07-L	5 x 5 μl (100 mM)	Sob demanda	Adicionar ao Carrinho
CLK-T07-XL	50 μl (100 mM)	Sob demanda	Adicionar ao Carrinho

For general laboratory use.

Envio: shipped on gel packs

Condições de armazenamento: store at -20 °C
Short term exposure (up to 1 week cumulative) to ambient temperature possible.

Validade: 12 months after date of delivery

Fórmula molecular: C11H15N2O14P3 (free acid)

Peso molecular: 492.16 g/mol (free acid)

Pureza: ≥ 95 % (HPLC)

Forma: solution in water

Concentração: 100 mM - 110 mM

pH: 7.5 ±0.5

Propriedades espectroscópicas: λmax 288 nm, ε 12.0 L mmol-1 cm-1 (Tris-HCl pH 7.5)

Formulários:
Incorporation into DNA by PCR with family B polymerases (Pwo, Deep Vent exo^- or KOD XL)^[1,2]
Incorporation into DNA by Primer Extension with family A polymerase Taq and family B polymerases (Pwo, Deep Vent exo^- or KOD XL)^[1]
The resulting ethynyl-functionalized DNA can subsequently be processed via Cu(I)-catalyzed click chemistry that offers the choice

to introduce a Biotin group (via Azides of Biotin) for subsequent purification tasks
to introduce fluorescent group (via Azides of fluorescent dyes) for subsequent microscopic imaging
to crosslink the DNA to Azide-functionalized biomolecules e.g.proteins

Presolski et al.^[3] and Hong et al.^[4] provide a general protocol for Cu(I)-catalyzed click chemistry reactions that may be used as a starting point for the set up and optimization of individual assays.

Produtos relacionados: Copper (II)-Sulphate (CuSO4), #CLK-MI004 Tris(3-hydroxypropyltriazolylmethyl)amine (THPTA), #CLK-1010 Sodium Ascorbate (Na-Ascorbate), #CLK-MI005

Referências selecionadas:
[1] Gierlich et al. (2007) Synthesis of Highly Modified DNA by a Combination of PCR with Alkyne-Bearing Triphosphates and Click Chemistry. Chem. Eur. J. 13:9486.

[2] Burley et al. (2006) Directed DNA Metallization. J. Am. Chem. Soc. 128 (5):1398.

[3] Presolski et al. (2011) Copper-Catalyzed Azide-Alkyne Click Chemistry for Bioconjugation. Current Protocols in Chemical Biology 3:153.

[4] Hong et al. (2011) Analysis and Optimization of Copper-Catalyzed Azide-Alkyne Cycloaddition for Bioconjugation. Angew. Chem. Int. Ed. 48:9879.