pCp-Cy5
Cytidine-5'-phosphate-3'-(6-aminohexyl)phosphate, labeled with Cy5, Triethylammonium salt
| Catálogo Nº | Apresentação | Preço (R$) | Comprar / Observação |
|---|---|---|---|
| NU-1706-CY5 | 20 μl (1 mM) | Sob demanda | Adicionar ao Carrinho |
Excitation and Emission spectrum of Cy5
For general laboratory use.
Envio: shipped on gel packs
Condições de armazenamento: store at -20 °C
Short term exposure (up to 1 week cumulative) to ambient temperature possible.
Validade: 12 months after date of delivery
Fórmula molecular: C48H66N6O18P2S2 (free acid)
Peso molecular: 1141.14 g/mol (free acid)
Pureza: ≥ 95 % (HPLC)
Forma: solution in 10 mM Tris-HCl
Concentração: 1.0 mM - 1.1 mM
pH: 7.5 ±0.5
Propriedades espectroscópicas: λexc 649 nm, λem 670 nm, ε 250.0 L mmol-1 cm-1 (Tris-HCl pH 7.5)
Formulários:
Incorporation into RNA/miRNA by
- 3'-End Labeling with T4 RNA Ligase[1]
Descrição:
pCp-Cy5 is recommended for 3'-End Labeling of RNA/miRNA with T4 RNA Ligase that conjugates a single dye molecule to the 3'-OH group of the RNA/miRNA template. The resulting Dye-labeled RNA/miRNA probes are ideally suited for hybridization experiments such as microarray-based expression profiling or electro mobility shift assays (EMSA).
Please note: Protect the Dye-labeled pCp from exposure to light and carry out experimental procedures under low light conditions.
Produtos relacionados: pCp-BIO, #NU-1706-BIO pCp-Desthiobiotin, #NU-1706-Desthiobio pCp-Cy3, #NU-1706-CY3 pCp-Amine, #NU-1706 pCp-Azide, #NU-1708 pCp-Alkyne, #NU-1709
Referências selecionadas:
[1] Wang et al. (2007) Direct and sensitive miRNA profiling from low-input total RNA. RNA 13 (1):151.