5-Propargylamino-dCTP-ATTO-647N
5-Propargylamino-2'-deoxycytidine-5'-triphosphate, labeled with ATTO 647N, Triethylammonium salt
| Catálogo Nº | Apresentação | Preço (R$) | Comprar |
|---|---|---|---|
| NU-809-647N-S | 10 μl (1 mM) | Sob demanda | Adicionar ao Carrinho |
| NU-809-647N-L | 5 x 10 μl (1 mM) | Sob demanda | Adicionar ao Carrinho |
excitation and emission spectrum of ATTO 647N
For general laboratory use.
Envio: shipped on gel packs
Condições de armazenamento: store at -20 °C
Short term exposure (up to 1 week cumulative) to ambient temperature possible.
Validade: 12 months after date of delivery
Fórmula molecular: C54H68N7O15P3 (free acid)
Peso molecular: 1148.08 g/mol (free acid)
Pureza: ≥ 95 % (HPLC)
Forma: solution in water
Concentração: 1.0 mM - 1.1 mM
pH: 7.5 ±0.5
Propriedades espectroscópicas: λexc 646 nm, λem 664 nm, ε 150.0 L mmol-1 cm-1 (Tris-HCl pH 7.5)
Descrição:
5-Propargylamino-dCTP-ATTO-647N is recommended for direct enzymatic labeling of DNA/cDNA by Nick Translation. It is incorporated as substitute for its natural counterpart dCTP. The resulting Dye-labeled DNA/cDNA probes are ideally suited for fluorescence hybridization applications such as FISH or microarray-based gene expression profiling. Optimal substrate properties and thus labeling efficiency is ensured by an optimized linker attached to the C5 position of cytidine. Recommended Propargylamino-dCTP-ATTO-647N/dCTP ratio for Nick Translation: 30-50% Propargylamino-dCTP-ATTO-647N/ 70-50% dCTP Please note: Protect the Dye-labeled dCTP from exposure to light and carry out experimental procedures in low light conditions. The optimal final concentration of the Dye-labeled dCTP may very depending on the application and assay conditions. For optimal product yields and high incorporation rates an individual optimization of the Dye-labeled-dCTP/dCTP ratio is recommended.