Biotin-11-UTP - high concentration

For general laboratory use.

Envio: shipped on gel packs

Condições de armazenamento: store at -20 °C
Short term exposure (up to 1 week cumulative) to ambient temperature possible.

Validade: 12 months after date of delivery

Fórmula molecular: C28H45N6O18P3S (free acid)

Peso molecular: 878.67 g/mol (free acid)

Pureza: ≥ 95 % (HPLC)

Forma: filtered solution (30 kDa) in 20 mM Tris-HCl

Concentração: 75 mM - 80 mM

pH: 7.5 ±0.5

Propriedades espectroscópicas: λmax 289 nm, ε 7.1 L mmol-1 cm-1 (Tris-HCl pH 7.5)

Formulários:
Microarray based hybridization detection^{[1, 2]}
RNA-labeling^[2]
Biotin-PAGE^[3]
ISH^[4]

Referências selecionadas:
[1] Lonergan et al. (2007) Comparison of target labeling methods for use of Affymetrix GeneChips. BMC Biotechnology 7:24.

[2] Glaid et al. (1989) Non-isotopic RNA probes. Comparison between different labels and detection systems. Histochemistry 93:91.

[3] Theissen et al. (1989) Degree of biotinylation in nucleic acids estimated by a gel retardation assay. Analyt. Biochem. 179:98.

[4] Baumann et al. (1988) Flow cytometric detection of ribosomal RNA in suspended cells by fluorescent in situ hybridization. Cytometry 9:517.