Aminoallyl-dUTP-XX-Cy3
5-(3-Aminoallyl)-2'-deoxyuridine-5'-triphosphate, labeled with Cy3, Triethylammonium salt
| Catálogo Nº | Apresentação | Preço (R$) | Comprar |
|---|---|---|---|
| NU-803-XX-CY3-S | 10 μl (1 mM) | Sob demanda | Adicionar ao Carrinho |
| NU-803-XX-CY3-L | 5 x 10 μl (1 mM) | Sob demanda | Adicionar ao Carrinho |
Excitation and Emission spectrum of Cy3
For general laboratory use.
Envio: shipped on gel packs
Condições de armazenamento: store at -20 °C
Short term exposure (up to 1 week cumulative) to ambient temperature possible.
Validade: 12 months after date of delivery
Fórmula molecular: C55H78N7O23P3S2 (free acid)
Peso molecular: 1362.29 g/mol (free acid)
Pureza: ≥ 95 % (HPLC)
Forma: filtered solution (30 kDa) in 10 mM Tris-HCl
Concentração: 1.0 mM - 1.1 mM
pH: 7.5 ±0.5
Propriedades espectroscópicas: λexc 550 nm, λem 570 nm, ε 150.0 L mmol-1 cm-1 (Tris-HCl pH 7.5), CF260 0.08
Formulários:
Incorporation into DNA/cDNA by
- PCR with Taq polymerase in-house data
- Nick Translation with DNAse I/ DNA Polymerase I in-house data
Descrição:
Aminoallyl-dUTP-XX-Cy3 is recommended for direct enzymatic labeling of DNA/cDNA e.g. by PCR and Nick Translation. It is incorporated as substitute for its natural counterpart dTTP. The resulting Dye-labeled DNA/cDNA probes are ideally suited for fluorescence hybridization applications such as FISH or microarray-based gene expression profiling. Optimal substrate properties and thus labeling efficiency is ensured by an optimized linker attached to the C5 position of uridine. Higher incorporation and thus labeling rates can be achieved compared to Aminoally-dUTP-Cy3 (NU-803-Cy3).
Recommended Aminoallyl-dUTP-XX-Cy3/dTTP ratio for PCR and Nick Translation: 30-50% Aminoallyl-dUTP-XX-Cy3/ 50% dTTP
Please note: Protect the Dye-labeled dUTP from exposure to light and carry out experimental procedures in low light conditions. The optimal final concentration of the Dye-labeled dUTP may very depending on the application and assay conditions. For optimal produdct yields and high incorporation rates an individual optimization of the Dye-labeled-dUTP/dTTP ratio is recommended.
Produtos relacionados:
- HighFidelity Cy3 PCR Labeling Kit, #APP-101-Cy3
- HighFidelity YELLOW PCR Labeling Testkit, #APP-101-YELLOW
- Cy3 NT Labeling Kit, #PP-305-Cy3