Aminoallyl-dUTP-ATTO-647N
5-(3-Aminoallyl)-2'-deoxyuridine-5'-triphosphate, labeled with ATTO 647N, Triethylammonium salt
| Catálogo Nº | Apresentação | Preço (R$) | Comprar |
|---|---|---|---|
| NU-803-647N-S | 10 μl (1 mM) | Sob demanda | Adicionar ao Carrinho |
| NU-803-647N-L | 5 x 10 μl (1 mM) | Sob demanda | Adicionar ao Carrinho |
excitation and emission spectrum of ATTO 647N
For general laboratory use.
Envio: shipped on gel packs
Condições de armazenamento: store at -20 °C
Short term exposure (up to 1 week cumulative) to ambient temperature possible.
Validade: 12 months after date of delivery
Fórmula molecular: C58H78N9O15P3 (free acid)
Peso molecular: 1151.08 g/mol (free acid)
Pureza: ≥ 95 % (HPLC)
Forma: filtered solution (30 kDa) in 10 mM Tris-HCl
Concentração: 1.0 mM - 1.1 mM
pH: 7.5 ±0.5
Propriedades espectroscópicas: λexc 646 nm, λem 664 nm, ε 150.0 L mmol-1 cm-1 (Tris-HCl pH 7.5)
Formulários:
Incorporation into DNA/cDNA by
- Nick Translation with DNAse I/ DNA Polymerase I in-house data
Descrição:
Aminoallyl-dUTP-ATTO647N is recommended for direct enzymatic labeling of DNA/cDNA by Nick Translation. It is incorporated as substitute for its natural counterpart dTTP. The resulting Dye-labeled DNA/cDNA probes are ideally suited for fluorescence hybridization applications such as FISH or microarray-based gene expression profiling. Optimal substrate properties and thus labeling efficiency is ensured by an optimized linker attached to the C5 position of uridine.
Recommended Aminoallyl-dUTP-ATTO647N/dTTP ratio for Nick Translation: 30-50% Aminoallyl-dUTP-ATTO647N/ 50% dTTP
Please note: Protect the Dye-labeled dUTP from exposure to light and carry out experimental procedures in low light conditions. The optimal final concentration of the Dye-labeled dUTP may very depending on the application and assay conditions. For optimal produdct yields and high incorporation rates an individual optimization of the Dye-labeled-dUTP/dTTP ratio is recommended.