Marcação DNA/RNA e Bio Moléculas

5-Propargylamino-dCTP - Solution

5-Propargylamino-2'-deoxycytidine-5'-triphosphate, Sodium salt

Catálogo Nº	Apresentação	Preço (R$)	Comprar
NU-809S	10 μl (100 mM)	Sob demanda	Adicionar ao Carrinho
NU-809L	5 x 10 μl (100 mM)	Sob demanda	Adicionar ao Carrinho
NU-809XL	100 μl (100 mM)	Sob demanda	Adicionar ao Carrinho

For general laboratory use.

Envio: shipped on gel packs

Condições de armazenamento: store at -20 °C
Short term exposure (up to 1 week cumulative) to ambient temperature possible.

Validade: 12 months after date of delivery

Fórmula molecular: C12H19N4O13P3 (free acid)

Peso molecular: 520.22 g/mol (free acid)

CAS#: 115899-39-3

Pureza: ≥ 93 % (HPLC)

Forma: solution in water

Concentração: 100 mM - 110 mM

pH: 7.5 ±0.5

Propriedades espectroscópicas: λmax 294 nm, ε 9.3 L mmol-1 cm-1 (Tris-HCl pH 7.5)

Formulários:
Incorporation into DNA by

PCR with Taq polymerase^{in-house data}

Descrição:
Propargylamino-dCTP is recommended for two-step labeling of DNA e.g. by PCR. It is enzymatically incorporated into DNA as substitute for its natural counterpart dCTP. The resulting Amine-functionalized DNA can subsequently be labeled via the classic Amine/NHS Ester reaction that offers the choice to introduce a Biotin group (via NHS Ester of Biotin) for subsequent purification tasksto introduce fluorescent group (via NHS Ester of fluorescent dyes) for subsequent microscopic imaging