DBCO-PEG4-Biotin Conjugate

For general laboratory use.

Envio: shipped at ambient temperature

Condições de armazenamento: store at -20 °C
store undissolved, for use prepare a fresh solution

Validade: 12 months after date of delivery (undissolved)

Fórmula molecular: C39H51N5O8S

Peso molecular: 749.92 g/mol

CAS#: 1255942-07-4

Pureza: ≥ 90 % (HPLC)

Forma: off-white to slightly yellow solid at storage conditions, tends to become sticky amorphous solid

Solubilidade: DMF, DMSO

Descrição:
DBCO-PEG4-Biotin is suitable for the introduction of a biotin moiety to Azide-labeled biomolecules via Cu(I)-free strain-promoted Alkyne-Azide Click Chemistry (SPAAC) reaction.

The hydrophilic PEG4 linker [22.8 Å (19 atoms)] reduces or eliminates aggregation and precipitation during the labeling process by increasing the hydrophilicity of the target molecule. It furthermore enhances the accessibility of the biotin moiety and thus the detection efficiency of the biotinylated molecule via fluorescent or HRP-labeled streptavidin or its affinity purification via streptavidin agarose.

Due to the extremely high affinity of biotin towards streptavidin (KD = 10-15 M), the biotinylated molecule/streptavidin-interaction is essentially irreversible under physiological conditions.