5-Vinyl-2'-deoxyuridine (5-VdU)
5-Ethenyl-2'-deoxyuridine
2'-Deoxy-5-vinyluridine
2'-Deoxy-5-ethenyluridine
| Catálogo Nº | Apresentação | Preço (R$) | Comprar |
|---|---|---|---|
| CLK-050-10 | 10 mg | Sob demanda | Adicionar ao Carrinho |
| CLK-050-50 | 50 mg | Sob demanda | Adicionar ao Carrinho |
For general laboratory use.
Envio: shipped at ambient temperature
Condições de armazenamento: store at -20 °C
Short term exposure (up to 1 week cumulative) to ambient temperature possible.
Validade: 12 months after date of delivery
Fórmula molecular: C11H14N2O5
Peso molecular: 254.24 g/mol
CAS#: 55520-67-7
Pureza: ≥ 98 % (HPLC)
Forma: solid
Solubilidade: DMSO, methanol
Propriedades espectroscópicas: λmax 292 nm, ε 9.2 L mmol-1 cm-1 (Methanol)
Formulários:
DNA synthesis monitoring[1]
Descrição:
5-VdU (5-Vinyl-2'-deoxyuridine) can be used as a replacement for BrdU (5-Bromo-2'-deoxyuridine) or the copper-catalyst requiring 5-EdU (5-Ethynyl-2'-deoxyuridine) to measure de novo DNA synthesis during the S-phase of the cell cycle.
5-VdU is cell permeable and incorporates into replicating DNA instead of its natural analog thymidine. The resulting vinyl-functionalized DNA can subsequently be detected via Cu(I)-free Alkene-Tetrazine Ligation that offers the choice to introduce a Biotin group (via Tetrazines of Biotin) for subsequent purification tasks or a fluorescent group (via Tetrazines of fluorescent dyes) for subsequent microscopic imaging [1].
Produtos relacionados: 5-Ethynyl-2'-deoxy-uridine (5-EdU), #CLK-N001 5-Vinyl-uridine (5-VU), #CLK-049
Referências selecionadas:
[1] Rieder et al. (2014) Alkene-Tetrazine Ligation for Imaging Cellular DNA. Angew. Chem. Int. Ed. 53:9168.