5-TCO-PEG4-dUTP
5-trans-Cyclooctene-PEG4-dUTP
| Catálogo Nº | Apresentação | Preço (R$) | Comprar |
|---|---|---|---|
| CLK-035S | 200 μl (1 mM) | Sob demanda | Adicionar ao Carrinho |
| CLK-035L | 5 x 200 μl (1 mM) | Sob demanda | Adicionar ao Carrinho |
For general laboratory use.
Envio: shipped on gel packs
Condições de armazenamento: store at -20 °C
Short term exposure (up to 1 week cumulative) to ambient temperature possible.
Validade: 12 months after date of delivery
Fórmula molecular: C32H53N4O21P3 (free acid)
Peso molecular: 922.70 g/mol (free acid)
Pureza: ≥ 95 % (HPLC)
Forma: solution in water
Concentração: 1.0 mM - 1.1 mM
pH: 7.5 ±0.5
Propriedades espectroscópicas: λmax 240 nm, ε 10.7 L mmol-1 cm-1 (Tris-HCl pH 7.5)
Formulários:
Incorporation into DNA/cDNA by
- PCR with Taq polymerase in-house data
Descrição:
5-TCO-dUTP is recommended for two-step labeling of DNA/cDNA e.g. by PCR. It is enzymatically incorporated into DNA/cDNA as substitute for its natural counterpart dTTP. The resulting TCO-functionalized DNA/cDNA can subsequently be labeled via Cu(I)-free Click Chemistry (TCO-Tetrazine-Ligation) that offers the choice
to introduce a Biotin group (via Tetrazines of Biotin) for subsequent purification tasksto introduce fluorescent group (via Tetrazines of fluorescent dyes) for subsequent microscopic imagingto crosslink the DNA to Tetrazine-functionalized biomolecules e.g. proteins