DBCO-PEG4-FLAG

For general laboratory use.

Envio: shipped on gel packs

Condições de armazenamento: store at -20 °C
Short term exposure (up to 1 week cumulative) to ambient temperature possible.

Validade: 12 months after date of delivery

Fórmula molecular: C80H107N15O30S

Peso molecular: 1790.85 g/mol

Pureza: ≥ 95 % (HPLC)

Forma: solid

Solubilidade: water, PBS (up to 30 mM tested)

Propriedades espectroscópicas: λmax 308 nm, ε 13.8.0 L mmol-1 cm-1

Descrição:
DBCO-PEG4-FLAG enables the FLAG-tag (DYKDDDDK) attachment to any Azide-functionalized molecule via Cu(I)-free strain-promoted Alkyne-Azide Click Chemistry (SPAAC). The resulting FLAG-tagged molecules can subsequently be detected by an anti-FLAG antibody that is either immobilized onto a matrix (for purification) or coupled with a fluorescent dye or reporter enzyme for direct or indirect detection, respectively. Molecule solubility and efficient FLAG-tag detection is ensured by the integrated PEG-linker.

DBCO-FLAG (without PEG-linker) has been successfully used for the detection of cell-surface Azides (final concentration: 100 μM)[1] or labeling of Azide-functionalized micelles (amount: 100 nmol)[2]. These concentrations may serve as a starting point for individual assay set-up.

Produtos relacionados: Azide-PEG3-FLAG, #CLK-032

Referências selecionadas:
[1] Patterson et al. (2014) Improved cyclopropene reporters for probing protein glycosylation. Molecular BioSystems 10:1693.

[2] Chan et al. (2013) Double Click: Dual Functionalized Polymeric Micelles with Antibodies and Peptides. Bioconjugate Chemistry 24:105.