UDP-GalNAz

For general laboratory use.

Envio: shipped at ambient temperature

Condições de armazenamento: store at -20 °C
Short term exposure (up to 1 week cumulative) to ambient temperature possible.

Validade: 12 months after date of delivery

Fórmula molecular: C17H26N6O17P2 (free acid)

Peso molecular: 648.37 g/mol (free acid)

Pureza: ≥ 95 % (HPLC)

Forma: solid

Solubilidade: water, aqueous buffer pH 7-7.5

Propriedades espectroscópicas: λmax 262 nm, ε 10.0 L mmol-1 cm-1 (Tris-HCl pH 7.5)

Formulários:
Transfer of GalNAz moiety to GalNAc residues of proteins and monoclonal antibodies with ß1,4-galactosyltranferase mutant Y289L-Gal-T1 ^[1-2].
The azide-functionalized target can subsequently be detected via Cu(I)-catalyzed or Cu(I)-free Click Chemistry that offers the choice to introduce

• a Biotin group (via Azides of Biotin or DBCO-containing Biotin, respectively) for subsequent purification tasks or
• a fluorophore (via Azides of fluorescent dyes or DBCO-containing fluorescent dyes, respectively) for subsequent microscopic imaging

Produtos relacionados:

• UDP-6-azide-glucose, #CLK-076
• Ac4GalNAz, #CLK-1086

Referências selecionadas:
[1] Hang et al. (2004) Probing glycosyltransferase activities with the Staudinger ligation. J. Am. Chem. Soc. 126(1):6.

[2] Qasba et al. (2008) Site-specific linking of biomolecules via glycan residues using glycosyltransferases. Biotechnol. Prog. 24(3):520.