JBS Deep Purple

For general laboratory use.

Envio: shipped at ambient temperature

Condições de armazenamento: store at ambient temperature

Validade: 12 months

Fórmula molecular: C25H30N3Cl

Peso molecular: 407.98 g/mol

CAS#: 548-62-9

Número CE: 208-953-6

Concentração: 0.5 mM

Formulários:
JBS Deep Purple is a crystal dye used to stain macromolecular crystals, i.e. protein, peptide and nucleic acid crystals in order to differentiate them from small molecules and salt crystals.

Descrição:
Crystallization screening with high concentrations of precipitant and salt may lead to the formation of salt crystals. It is quite difficult to make a distinction between these false positives and true protein crystals.

Staining of crystals with appropriate dyes is a very straightforward method to differentiate between macromolecular crystals and salt crystals [1].

Protein and salt crystals differ substantially in their solvent content. Small crystal dyes, like JBS Deep Purple, are able to permeate the solvent channels of a protein, thus coloring the protein purple. In contrast, salt crystals are tightly packed and do not possess large solvent channels. They will therefore remain colourless.

Usage:
Simply add 0.5-1 μl of JBS Deep Purple to the crystallization drop containing the crystals of interest.

Coloring Time:
JBS Deep Purple colors protein crystals after a few minutes. Even if the color of the solution is only faintly purple under the microscope, proteins will be stained within 5-15 min.
Very occasionally, it has been reported that protein crystals did not absorb crystal dyes [2].

Referências selecionadas:
[1] Wilkosz et al. (1995) Preliminary characterization of EcoRI-DNA co-crystals: incomplete factorial design of oligonucleotide sequences. Acta Cryst. D 51:938.
[2] Eckert et al. (2003) Crystallization and preliminary X-ray analysis of Alicyclobacillus acidocaldarius endoglucanase CelA. Acta Cryst. D 59:139.