JBS True Blue
Methylene Blue hydrated
| Catálogo Nº | Apresentação | Preço (R$) | Comprar / Observação |
|---|---|---|---|
| CO-301 | 300 μl | Sob demanda | Adicionar ao Carrinho |
Unstained (A) and stained (B) protein crystals
For general laboratory use.
Envio: shipped at ambient temperature
Condições de armazenamento: store at ambient temperature
Validade: 12 months
Fórmula molecular: C16H18ClN3S * H2O
Peso molecular: 319.85 g/mol (anhydrous)
CAS#: 61-73-4
Número CE: 200-515-2
Formulários:
JBS True Blue is a crystal dye used to stain macromolecular crystals, i.e. protein, peptide and nucleic acid crystals in order to differentiate them from small molecules and salt crystals.
Descrição:
Crystallization screening with high concentrations of precipitant and salt may lead to the formation of salt crystals. It is quite difficult to make a distinction between these false positives and true protein crystals.
Staining of crystals with appropriate dyes is a very straightforward method to differentiate between macromolecular crystals and salt crystals [1].
Protein and salt crystals differ substantially in their solvent content. Small crystal dyes, like JBS True Blue, are able to permeate the solvent channels of a protein, thus coloring the protein blue. In contrast, salt crystals are tightly packed and do not possess large solvent channels. They will therefore remain colourless.
Usage:
Simply add 0.5 μl of JBS True Blue to the crystallization drop containing the crystals of interest.
Coloring Time:
JBS True Blue colors protein crystals after a few minutes. Even if the color of the solution is only faintly blue under the microscope, proteins will be stained within 5-15 min.
Very occasionally, it has been reported that protein crystals did not absorb crystal dyes [2].
Safety Information:
Methylene Blue is a harmful substance if swallowed. Work carefully and wear gloves when handling the dye. Under fire conditions it emits toxic fumes.
Referências selecionadas:
[1] Wilkosz et al. (1995) Preliminary characterization of EcoRI-DNA co-crystals: incomplete factorial design of oligonucleotide sequences. Acta Cryst. D 51:938.
[2] Eckert et al. (2003) Crystallization and preliminary X-ray analysis of Alicyclobacillus acidocaldarius endoglucanase CelA. Acta Cryst. D 59:139.