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  2. Marcação DNA/RNA e Bio Moléculas
  3. Tecnologias para RNA
  4. Análise & Detecção de RNA
  5. Imagem & Análise de RNA com base em Aptâmero

Imagem & Análise de RNA com base em Aptâmero

Propriedades espectroscópicas de complexos aptâmeros/dyes

DyeLight-up aptamerExc.max/Em.max [nm]
of aptamer/dye complex		ɛmax [M-1cm-1]
of aptamer/dye complex		Reference
DFHBISpinach469/50124271[5]
Spinach 2447/50122000[6]
DFHBI-1TSpinach 2482/50531000[6]
Broccoli472/50729600[7]
DFHOCorn505/54529000[8]

Produtos

DFHBI RNT-001

Mimic of green fluorescent protein (GFP) fluorophore  

DFHBI-1T RNT-002

Mimic of green fluorescent protein (GFP) fluorophore  

DFHO RNT-003

Mimic of red fluorescent protein (RFP) fluorophore  

Referências Selecionadas

[1] Neubacher et al. (2019) RNA Structure and Cellular Applications of Fluorescent Light-Up Aptamers. Angew. Chem. Int. Ed. 58:1266.
[2] Ouellet (2016) RNA Fluorescence with Ligth-Up Aptamers. Front. Chem 4:29.
[3] George et al. (2018) Intracelluar RNA-tracking methods. Open Biol 8:180104.
[4] Dologosheina et al. (2016) Fluorophore Binding RNA aptamers and their application. Wires RNA 7(6):843.
[5] Paige et al. (2011) RNA mimics of green fluorescent protein. Science 333(6042):642.
[6] Song et al. (2014) Plug-and-Play fluorophores extend the Spectral Properties of Spinach. J. Am. Chem. Soc. 136:1198.
[7] Filonov et al. (2014) Broccoli: Rapid Selection of an RNA Mimic of Green Fluorescent Protein by Fluorescence-Based Selection and Directed Evolution. J. Am. Chem. Soc. 136:16299.
[8] Song et al. (2017) Imaging RNA polymerase III transcription using a photostable RNA-fluorophore complex. Nat. Chem. Biol. 13(11):1187.