TEV Protease
Catálogo Nº | Apresentação | Preço (R$) | Comprar |
---|---|---|---|
ENZ-102S | 1.000 U | 491,40 | Adicionar ao Carrinho |
ENZ-102M | 10.000 U | 3.276,00 | Adicionar ao Carrinho |
ENZ-102L | 50.000 U | 12.285,00 | Adicionar ao Carrinho |
For in vitro use only!
Definição de unidade: One unit of TEV protease cleaves >85% of 3 μg of control substrate in one hour at pH 8.0 at 30 °C.
Envio: Shipped on blue ice
Condições de armazenamento: Store at -20 °C
Validade: 12 months
Concentração: 0.5 mg/mL (5 U/μL)
Descrição:
Turbo TEV Protease is an enhanced form of Tobacco Etch Virus (TEV) protease that is highly site-specific, active, and more stable than native TEV protease. Turbo TEV Protease recognizes the seven-amino-acid sequence Glu-Asn-Leu-Tyr-Phe-Gln-Gly and cleaves between Gln and Gly with high specificity. The protease is used to cleave affinity tags from fusion proteins. The optimal temperature for cleavage is 30°C; however, the enzyme is active over wide ranges of temperature and pH (pH 6.0-8.5). Following digestion, Turbo TEV Protease is easily removed from the cleavage reaction by affinity chromatography using the polyhistidine tag at the N-terminus of the protease.
Removal of Turbo TEV Protease after Cleavage
The Turbo TEV protease contains a polyhistidine tag at the Nterminus. After cleavage of the fusion protein, remove Turbo TEV Protease from the cleavage reaction by affinity chromatography on a nickel chelating resin.