TEV Protease

Catálogo Nº Apresentação Preço (R$) Comprar
ENZ-102S 1.000 U491,40 Adicionar ao Carrinho
ENZ-102M 10.000 U3.276,00 Adicionar ao Carrinho
ENZ-102L 50.000 U12.285,00 Adicionar ao Carrinho

For in vitro use only!

Definição de unidade: One unit of TEV protease cleaves >85% of 3 μg of control substrate in one hour at pH 8.0 at 30 °C.

Envio: Shipped on blue ice

Condições de armazenamento: Store at -20 °C

Validade: 12 months

Concentração: 0.5 mg/mL (5 U/μL)

Descrição:
Turbo TEV Protease is an enhanced form of Tobacco Etch Virus (TEV) protease that is highly site-specific, active, and more stable than native TEV protease. Turbo TEV Protease recognizes the seven-amino-acid sequence Glu-Asn-Leu-Tyr-Phe-Gln-Gly and cleaves between Gln and Gly with high specificity. The protease is used to cleave affinity tags from fusion proteins. The optimal temperature for cleavage is 30°C; however, the enzyme is active over wide ranges of temperature and pH (pH 6.0-8.5). Following digestion, Turbo TEV Protease is easily removed from the cleavage reaction by affinity chromatography using the polyhistidine tag at the N-terminus of the protease.

Removal of Turbo TEV Protease after Cleavage
The Turbo TEV protease contains a polyhistidine tag at the Nterminus. After cleavage of the fusion protein, remove Turbo TEV Protease from the cleavage reaction by affinity chromatography on a nickel chelating resin.