Proteinase K - Solution

from Tritirachium album

Endopeptidase K

Catálogo Nº Apresentação Preço (R$) Comprar
EN-178S 2 x 1 mlSob demanda Adicionar ao Carrinho
EN-178L 10 x 1 mlSob demanda Adicionar ao Carrinho

For general laboratory use.

Definição de unidade: One unit is the amount of enzyme which releases at 37 °C in 1 min as many folin-positive amino acids and peptides from haemoglobin as 1 μmol of tyrosine.

Envio: shipped on gel packs

Condições de armazenamento: store at -20 °C
avoid freeze/thaw cycles

Validade: 12 months

Peso molecular: 28.9 kDa

CAS#: 39450-01-6

Número CE: 254-457-8

Pureza: free of RNases, DNases and Exonucleases

Forma: Proteinase K solution in 10 mM Tris-HCl, 1 mM Ca(H3C2O2)2, 10 % (v/v) Glycerol, pH 7.8 (22°C)

Concentração: 20 mg/ml

Atividade:
> 600 units/ml

Formulários:
Digestion of proteins during DNA and RNA preparation.

Descrição:
Proteinase K is a serine protease that exhibits a very broad cleavage specificity. The Protein with a molecular weight of 28.9 kDa cleaves peptide bonds adjacent to the carboxylic group of aliphatic and aromatic amino acids. Proteinse K is not inactivated by metal chelating reagents such as EDTA or detergents such as SDS and is active over a wide range of pH (4 - 12.5).
Proteinase K is a highly active and stable protease with low cutting specificity. The enzyme belongs to the group of subtilisine-related serine proteases and is strongly inhibited by PMSF.
In presence of 0.5 - 1 % SDS Proteinase K inactivates DNases and RNases in eucaryotic and microbiological cell cultures. The use of Proteinase K during lysis of the cells allows the isolation of intact highly-molecular nucleic acids.