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  5. Turbonuclease

Turbonuclease

Encoded by the same gene of Benzonase®

Datasheet (PDF)
Catálogo Nº Apresentação Preço (R$) Comprar
ENZ-103S 5.000 unidades282,98 Adicionar ao Carrinho
ENZ-103M 25.000 unidades1.085,70 Adicionar ao Carrinho
ENZ-103L 2 x 25.000 unidades1.732,50 Adicionar ao Carrinho
ENZ-103XL 500.000 unidades13.860,00 Adicionar ao Carrinho

For in vitro use only!

Definição de unidade: One unit will digest sonicated salmon sperm DNA to acid-soluble oligonucleotides equivalent to a Δ260 of 1.0 in 30 min at pH 8.0 at 37 °C.

Envio: shipped on blue ice

Condições de armazenamento: store at -20 °C
avoid freeze/thaw cycles

Validade: 12 months

Forma: liquid (Supplied in 20 mM Tris-HCl pH 8.0, 20 mM NaCl, 2 mM MgCl2, 1 mM DTT and 50 % [v/v] glycerol)

Concentração: 250 units/μl

Formulários:
Turbo Nuclease is very effective in degrading nucleic acid from protein samples and it is used in a variety of application where complete hydrolysis of DNA/RNA is required:

  • Reduction of viscosity from cell lysates
  • Prevention of cell clumping
  • Elimination of unspecific protein-DNA complexes prior 2D- or native gel electrophoresis
  • Removal of nucleic acids from large-scale protein preparations

Descrição:
Turbo Nuclease is a broad-spectrum endonuclease that cleaves both DNA and RNA molecules independently of being single- or double-stranded, circular, linear or supercoiled. The enzyme is highly stable and active in a broad range of pH and temperature, making it ideal for a variety of downstream processes that require the degradation of DNA/RNA in a simple, efficient and specific manner.

  • Endonuclease from Serratia macescens recombinant expressed and purified from E. coli
  • Catalytic activity from pH 6 to 10 (optimal around 8.8) and temperature 0 to 44 °C
  • High catalytic efficiency (34-fold greater than DNase I)
  • Activity requires the presence of Mg2+ (optimum 2 mM)

Procedure:

  • Make a fresh, cold lysis buffer in which the target protein is soluble and is compatible with downstream purification processes, e.g. minimal amount of EDTA or DTT if a Ni-NTA column will be used.
  • Resuspend the thawed cell paste in lysis buffer. Use 2-10 ml Lysis Buffer for each gram of cell paste.
  • Add Turbo Nuclease to 2.5 units/ml.
  • A fluid 'aqueous' solution will result after 15 min.

Referências selecionadas:
Benedik et al. (1998) Serratia marcescens and its extracellular nuclease. FEMS Microbiol. Lett. 165 (1):1.