T4 dNMP (deoxy-Nucleotide Monophosphate) Kinase

bacteriophage T4, recombinant, E. coli

Catálogo Nº Apresentação Preço (R$) Comprar / Observação
PR-340 100 μg Sob demanda Adicionar ao Carrinho

For general laboratory use.

Envio: shipped on gel packs

Condições de armazenamento: store at -20 °C
avoid freeze/thaw cycles

Validade: 12 months

Peso molecular: 27 kDa

Número de acesso: AAD42414

Número de acesso: AAD42414

Pureza: > 90 % (SDS-PAGE)

Forma: liquid (Supplied in 100 mM Tris-HCl pH 7.5, 10 mM MgCl2 and 50 % glycerol)

Atividade: One unit of enzyme catalyses the phosphorylation of 1 nmol dGMP to dGDP per minute at 37°C. Specific activity: 100 u/μg.

Descrição:
NMP kinases catalyse the phosphorylation of nucleotide monophosphates resulting in the corresponding nucleotide diphosphates by using ATP as a phosphate donor. T4 dNMP kinase is the only member of the family of NMP kinases that recognizes three structurally dissimilar nucleotides: dGMP, dTMP and 5-hydroxymethyl-dCMP (5-OH-dCMP).

Activity Assay: 1 μg T4 dNMP kinase, 2 mM ATP and 2 mM dGMP in 100 mM Tris-HCl pH 7.5, 10 mM MgCl2.

Referências selecionadas:
Teplyakov et al. (1996) Crystal structure of bacteriophage T4 deoxynucleotide kinase with its substrates dGMP and ATP. EMBO J. 15:3487. Brush et al. (1993) Chemical modification of bacteriophage T4 deoxynucleotide kinase. Evidence of a single catalytic region. J. Biol. Chem. 268:1603. Brush et al. (1990) Bacteriophage T4 deoxynucleotide kinase: gene cloning and enzyme purification. J. Bacteriol. 172:2935.