dNTP set 100 mM

4 x 100 mM (dATP, dCTP, dGTP e dTTP)

2'-Deoxyadenosine-5'-triphosphate, Sodium salt; 2'-Deoxycytidine-5'-triphosphate, Sodium salt; 2'-Deoxyguanosine-5'-triphosphate, Sodium salt; 2'-Deoxythymidine-5'-triphosphate, Sodium salt

Catálogo Nº Apresentação Preço (R$) Comprar
NUC-102S 4 x 250 μL446,25 Adicionar ao Carrinho
NUC-102M 4 x 400 μL714,00 Adicionar ao Carrinho
NUC-102L 4 x 1000 μL1.610,70 Adicionar ao Carrinho
Structural formula of dNTP set 100 mM (4 x 100 mM (dATP, dCTP, dGTP e dTTP), 2'-Deoxyadenosine-5'-triphosphate, Sodium salt; 2'-Deoxycytidine-5'-triphosphate, Sodium salt; 2'-Deoxyguanosine-5'-triphosphate, Sodium salt; 2'-Deoxythymidine-5'-triphosphate, Sodium salt)
Structural formula of dNTP set 100 mM

For in vitro use only!

Envio: shipped on blue ice

Condições de armazenamento: store at -20 °C
Short term exposure (up to 1 week cumulative) to ambient temperature possible.

Validade: 12 months after date of delivery

Fórmula molecular:
dATP: C10H16N5O12P3 (free acid)
dCTP: C9H16N3O13P3 (free acid)
dGTP: C10H16N5O13P3 (free acid)
dTTP: C10H17N2O14P3 (free acid)

Peso molecular:
dATP: 491.18 g/mol (free acid)
dCTP: 467.15 g/mol (free acid)
dGTP: 507.18 g/mol (free acid)
dTTP: 482.17 g/mol (free acid)

Pureza: ≥ 99 % (HPLC)

Forma: clear aqueous solution

Concentração: 100 mM - 110 mM

pH: 8.5 ±0.2 (22 °C)

Propriedades espectroscópicas: dATP: λmax 259 nm, ε 15.4 L mmol-1 cm-1 (Tris-HCl pH 7.0) dCTP: λmax 271 nm, ε 8.9 L mmol-1 cm-1 (Tris-HCl pH 7.0) dGTP: λmax 252 nm, ε 13.7 L mmol-1 cm-1 (Tris-HCl pH 7.0) dTTP: λmax 262 nm, ε 9.6 L mmol-1 cm-1 (Tris-HCl pH 7.0)

Descrição:
dNTP Bundle contains four separate solutions of ultrapure dATP, dCTP, dGTP and dTTP supplied as clear aqueous solutions (pH 8.5).

dNTP Cat. No. cap color
dATP NU-1001 red
dCTP NU-1002 blue
dGTP NU-1003 yellow
dTTP NU-1004 green


Quality Control Specifications:
Low Copy Long Range PCR (18 kb, lambda DNA, template dilution series): PCR fragment with 50 pg of template or less
RT-PCR (749 bp fragment, human GAPDH gene, template dilution series): PCR fragment with 10 pg of template or less
Contamination with bacterial or human DNA: not detectable
DNases, RNases, Nicking Activity: not detectable
Proteases: not detectable

Referências selecionadas:
Erlich et al. (1988) Primer-directed enzymatic amplification of DNA with a thermostable DNA polymerase. Science 29 (239):487.
Holland et al. (1991) Detection of specific polymerase chain reaction product by utilizing the 5'----3' exonuclease activity of Thermus aquaticus DNA polymerase. Proc. Natl. Acad. Sci. USA 88 (16):7276.
Sanger et al. (1977) DNA sequencing with chain-terminating inhibitors. Proc. Natl. Acad. Sci. USA 74:5463.