Total RNA Purification Kit

Isolamento de RNA total por coluna de sílica com alta eficiência e rendimento

Catálogo Nº Apresentação Preço (R$) Comprar
DPK-108S 50 preps999,60 Adicionar ao Carrinho
DPK-108L 250 preps3.994,20 Adicionar ao Carrinho

For in vitro use only!

Envio: Shipped at ambient temperature

Condições de armazenamento: Store at ambient temperature

Validade: 12 months

Descrição:
Total RNA Purification Kit is designed for rapid, high purity and high yield isolation of total RNA from small amounts of various samples including blood, animal and plant tissue, bacteria and viruses. The spin column based method allows complete removal of inhibitors such as divalent cations and proteins. Due to elimination of phenol, handling of the kit is safe and no harmful waste is produced. The purified total RNA can be used in a number of downstream applications. The kit allows the purification of up to 100 μg RNA per preparation.

Contente:
• Lysis Buffer (before use, add 2-Mercaptoethanol as indicated on the bottle) - stable for 1 month at room temperature
• Activation Buffer
• Primary Washing Buffer (before use, add 96-99 % Ethanol as indicated on the bottle)
• Secondary Washing Buffer (before use, add 96-99 % Ethanol as indicated on the bottle)
• Elution Buffer
• Spin Columns
• 2 ml Collection Tubes

To be provided by you
• 2-Mercaptoethanol (2-ME)
• Optional: Chloroform
• 96-99 % Ethanol
• 2-Propanol (Isopropanol)
• 1.5 ml microtubes

Elimination of remaining DNA:
Remaining genomic DNA may be particularly a problem in subsequent RT-PCR or quantification of low-copy transcripts. For complete removal of gDNA from RNA preparations gDNA Removal Kit (Cat.-No. PP-219 from Jena Bioscience) is recommended.
The kit is based on a heat labile dsDNase which is irreversibly inactivated at moderate temperatures.