SYBR® Green Fluorescent DNA Stain

DNA intercalation dye for real-time PCR analysis

Catálogo Nº Apresentação Preço (R$) Comprar / Observação
PCR-378 500 μl x 100 μM Sob demanda Adicionar ao Carrinho
Excitation (blue) and emission (green) spectrum of SYBR® Green bound to dsDNA
Excitation (blue) and emission (green) spectrum of SYBR® Green bound to dsDNA

For general laboratory use.

Envio: shipped on gel packs

Condições de armazenamento: store at -20 °C
store dark

Validade: 12 months

Forma: liquid, supplied in 20 mM Tris-HCl pH 8.5, 0.1 mM EDTA and 0.01 % Tween-20

Concentração: 100 μM

Propriedades espectroscópicas: λexc 495 nm, λem 520 nm (bound to DNA)

Descrição:
SYBR® Green Fluorescent DNA Stain is a superior DNA intercalator dye specially developed for DNA analysis applications including real-time PCR (qPCR). Upon binding to DNA, the non-fluorescent dye becomes highly fluorescent while showing no detectable inhibition to the PCR process. The dye is extremely stable both thermally and hydrolytically, providing convenience during routine handling.

SYBR® Green Fluorescent DNA Stain is supplied as 100 μM concentration. Vortex SYBR® Green Fluorescent DNA Stain thoroughly prior to its use. An SYBR® Green concentration of 0.5-1.0 μM in the final assay is recommended. Add SYBR® Green Fluorescent DNA Stain as indicated in the table below per assay. Please note that the preparation of a master mix may be crucial in quantitative PCR reactions to reduce pipetting errors. Select the optical setting for SYBR® Green or FAM on the detection instrument. final SYBR® Green concentration||20 μl PCR assay||50 μl PCR assay 0.5 μM||0.1 μl||0.25 μl 1.0 μM||0.2 μl||0.50 μl SYBR® is a registered trademark of Invitrogen Corporation, Carlsbad, California, USA.