ROX Reference Dye
Corante de referência para normalização de sinal de fluorescência
| Catálogo Nº | Apresentação | Preço (R$) | Comprar |
|---|---|---|---|
| PCK-121S | 500 μL | 254,10 | Adicionar ao Carrinho |
| PCK-121L | 5 x 500 μL | 1.010,10 | Adicionar ao Carrinho |
Descrição:
ROX reference dye is optimized for block-based real-time PCR instruments that are compatible with the evaluation of the ROX reference signal. The dye does not take part in the PCR reaction but allows to normalize for non-PCR related signal variation and provides a baseline in multiplex reactions. The dye is supplied as 50x concentration.
Preparation of the qPCR master mix:
The preparation of a master mix is crucial in quantitative PCR reactions to reduce pipetting errors.
• Vortex the ROX reference dye thoroughly prior to addition to the master mix.
• Add 1 μL ROX reference dye for each 50 μL of master mix.