Taq Pol (-)MgCl2

DNA Polimerase, tampão sem MgCl2

Catálogo Nº Apresentação Preço (R$) Comprar
POL-114XS 250 U101,21 Adicionar ao Carrinho
POL-114S 500 U184,56 Adicionar ao Carrinho
POL-114M 1.000 U342,92 Adicionar ao Carrinho
POL-114L 2 x 1.000 U616,78 Adicionar ao Carrinho
POL-114XL 4 x 1.000 U1.107,35 Adicionar ao Carrinho

For in vitro use only!

Definição de unidade: One unit is defined as the amount of the enzyme required to catalyze the incorporation of 10 nmoles of dNTPs into an acid- insoluble form in 30 minutes at 70 °C.

Envio: Shipped on ice pack

Condições de armazenamento: Store at -20 °C
Avoid freeze/thaw cycles

Validade: 12 months

Concentração: 5 units/μL

Descrição:
Taq Pol is recommended for use in routine PCR reactions. It is optimized for high specificity and guarantees minimal by- product formation. The buffer system is recommended for plate based PCR and automated pipetting.
The enzyme replicates DNA at 72 °C. It catalyzes the polymerization of nucleotides into duplex DNA in 5' → 3' direction in the presence of magnesium. It also possesses a 5' → 3' polymerization-dependent exonuclease replacement activity but lacks a 3' → 5' exonuclease activity.

Contente:
Taq DNA Pol (blue cap) - POL - 100
5 units/μl Taq DNA Polymerase in Tris-HCl pH 8.0 (25 °C), KCl, EDTA, DTT,0.5%,50 (v/v)Glycerolandstabilizers.

Taq Reaction Buffer (-) Mg+2 (red cap) - 10x conc.
Tris-HCl pH 8.5 (25°C), KCl.

MgCl2 Stock Solution (yellow cap)
50 mM MgCl2