PCR Demo Kit - DNA Fragments

For in vitro use only!

Envio: shipped on blue ice

Condições de armazenamento: store at -20 °C
Short term storage (up to 1 month) at 4 °C possible.

Validade: 12 months

Forma: liquid

Concentração: 10x conc.

Formulários:
Taq Reaction Buffer complete (green cap)
200 mM Tris-HCl (pH 8.5 at 25 °C), 500 mM KCl and 15 mM MgCl₂.
Taq Reaction Buffer without MgCl₂ (blue cap)
200 mM Tris-HCl (pH 8.5 at 25 °C) and 500 mM KCl.
High Yield Buffer complete (green cap)
600 mM Tris-HCl (pH 8.8 at 25°C), 150 mM (NH₄)₂SO₄, 20 mM MgCl₂, 0.05 % Tween-20 and 0.05 % Nonidet P-40.
High Yield Buffer without MgCl₂ (blue cap)
600 mM Tris-HCl (pH 8.8 at 25°C), 150 mM (NH₄)₂SO₄, 0.05 % Tween-20 and 0.05 % Nonidet P-40.
Hot Start Buffer complete (green cap)
200 mM Tris-HCl (pH 8.5 at 25°C), 500 mM KCl and 15 mM MgCl₂.
Hot Start Buffer without MgCl₂ (blue cap)
200 mM Tris-HCl (pH 8.5 at 25°C) and 500 mM KCl.
MgCl₂ Stock Solution (yellow cap)
25 mM MgCl₂

Descrição:
PCR Reaction Buffers are ultrapure and sterile 10 x conc. reaction buffer for standard and routine PCR applications. MgCl₂ stock solution is recommended for individual optimization of for PCR assays.

Optimization of MgCl₂ concentration:
A concentration of 1.5 mM Mg²⁺ is recommended for most standard PCR applications. For an individual optimization use the reaction buffer without MgCl₂ and add MgCl₂ stock solution as shown in the table below.
20 μl PCR assay

50 μl PCR assay
2 conc.||1 mM||1.5 mM||2 mM||3 mM
Reaction Buffer without MgCl₂ - 10x||10 μl||10 μl||10 μl||10 μl
MgCl₂ Stock Solution - 25 mM||2 μl||3 μl||4 μl||6 μl