DNase Detection Kit

Detecção fluorescente da atividade de DNases

Catálogo Nº Apresentação Preço (R$) Comprar
PP-410S 50 reaçõesSob demanda Adicionar ao Carrinho
PP-410L 250 reaçõesSob demanda Adicionar ao Carrinho


For in vitro use only!

Envio: shipped on gel packs

Condições de armazenamento: store at -20 °C
store dark, avoid freeze/thaw cycles
stable at 4 °C for up to 4 weeks

Validade: 13 months

Forma: liquid

Descrição:
DNase Detection Kit provides a highly sensitive, fast and easy-to perform system for detection of DNase. The kit allows the detection of lowest amounts of ss- or ds-DNA degrading DNases. It is the ideal tool for contamination testing ranging from a few samples to routine process monitoring. The kit is based on a fluorescently labeled DNA probe exhibiting minimal fluorescence but showing a strong increase in fluorescence intensity in the presence of DNases. The probe is linked to the fluorophore FAM as reporter dye allowing excitation and detection with nearly all common real-time PCR cyclers or fluorescence readers.

Detection limit The detection limit of the assay is DNase I: < 1 x 10-5 units/μl ROX reference dye ROX Reference Dye does not take part in the detection reaction and allows therefore anormalization for non-RNase or -DNase related signal variations. We recommend to add ROX as internal standard if the instrument is compatible with the evaluation of the ROX reference signal. Spectroscopic data of FAM (DNase Probe) Excitation maximum: λEx = 495 nm Emission maximum: λEm = 520 nm Spectroscopic data of ROX (internal reference) Excitation maximum: λEx = 576 nm Emission maximum: λEm = 601 nm